Department of Geriatrics, Medical University of Lodz, 90-647 Lodz, Poland.
Department of Microbiology and Medical Laboratory Immunology, Medical University of Lodz, 90-213 Lodz, Poland.
Int J Environ Res Public Health. 2022 Mar 4;19(5):3009. doi: 10.3390/ijerph19053009.
In the event of blood culture contamination (BCC), blood culture (BC) needs to be repeated. This may delay appropriate treatment, prolong hospitalization and, consequently, increase its costs. The aim of the study was to assess the frequency of BCC and associated factors in a general hospital in Poland based on reports of BC in samples submitted for laboratory testing in 2019−2020. BCC is recognized when bacteria (especially those belonging to natural human microbiota) are isolated from a single sample and no clinical signs indicated infection. True positive BC is confirmed by the growth of bacteria in more than one set of blood samples with the corresponding clinical signs present. The structure of BC sets, microorganisms, and laboratory costs of BCC were analyzed. Out of 2274 total BC cases, 11.5% were true positive BC and 9.5% were BCC. Of all the BCC identified in the entire hospital, 72% was from Internal Medicine (IM) and Intensive Care Unit (ICU) combined. When single sets for BC were used in IM in 2020, the use increased to 85% compared with 2019 (p < 0.05). The predominant isolates were coagulase-negative staphylococci (84%). The estimated extra laboratory costs of BCC exceeded EUR 268,000. The BCC was a more serious problem than expected, including non-recommended using of single BC sets. Compliance with the BC collection procedure should be increased in order to reduce BCC and thus extra hospital costs.
如果发生血培养污染（BCC），则需要重复血培养（BC）。这可能会延迟适当的治疗，延长住院时间，并因此增加其成本。本研究旨在根据 2019-2020 年提交实验室检测的样本中 BC 的报告，评估波兰一家综合医院 BCC 的频率和相关因素。当从单个样本中分离出细菌（尤其是属于天然人体微生物群的细菌）且无感染临床症状时，即可认定为 BCC。真正的阳性 BC 通过在具有相应临床症状的多套血液样本中生长的细菌来确认。分析了 BC 集的结构、微生物和 BCC 的实验室成本。在 2274 例总 BC 病例中，11.5%为真正的阳性 BC，9.5%为 BCC。在整个医院中发现的所有 BCC 中，72%来自内科（IM）和重症监护病房（ICU）。当 2020 年内科仅使用一套 BC 时，与 2019 年相比，使用率增加到 85%（p < 0.05）。主要分离物是凝固酶阴性葡萄球菌（84%）。BCC 的额外实验室成本估计超过 268,000 欧元。BCC 的问题比预期的更为严重，包括不推荐使用单套 BC。为了减少 BCC 并降低医院的额外成本，应增加对 BC 采集程序的遵守。
