Lung resistance and elastance are different in ex vivo sheep lungs ventilated by positive and negative pressures.

Lung resistance () and elastance () can be measured during positive or negative pressure ventilation. Whether the different modes of ventilation produce different and is still being debated. Although negative pressure ventilation (NPV) is more physiological, positive pressure ventilation (PPV) is more commonly used for treating respiratory failure. In the present study, we measured lung volume, airway diameter, and airway volume, as well as and with PPV and NPV in explanted sheep lungs. We found that lung volume under a static pressure, either positive or negative, was not different. However, and were significantly higher in NPV at high inflation pressures. Interestingly, diameters of smaller airways (diameters <3.5 mm) and total airway volume were significantly greater at high negative inflation pressures compared with those at high positive inflation pressures. This suggests that NPV is more effective in distending the peripheral airways, likely due to the fact that negative pressure is applied through the pleural membrane and reaches the central airways via the peripheral airways, whereas positive pressure is applied in the opposite direction. More distension of lung periphery could explain why is higher in NPV (vs. PPV), because the peripheral parenchyma is a major source of tissue resistance, which is a part of the that increases with pressure. This explanation is consistent with the finding that during high frequency ventilation (>1 Hz, where reflects airway resistance more than tissue resistance), the difference in between NPV and PPV disappeared.