Waygood E B
Biochemistry. 1986 Jul 15;25(14):4085-90. doi: 10.1021/bi00362a015.
Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli has been reported to contain one phosphorylation site per dimer and thus operates by either a half of the sites or a flip-flop mechanism [Misset, O., & Robillard, G. T. (1982) Biochemistry 21, 3136-3142; Hoving, T., ten Hoeve-Duurkens, R., & Robillard, G. T. (1984) Biochemistry 23, 4335-4340]. In this paper, the determination of two phosphorylation sites per dimer of enzyme I was made by using a number of different methods. In some experiments, less than two sites per dimer were found, but a concomitant loss in enzyme I activity was also found. The phosphorylated residue in enzyme I was shown to have the properties expected for a N3-phosphohistidinyl residue.
据报道，大肠杆菌磷酸烯醇丙酮酸：糖磷酸转移酶系统的酶I每个二聚体含有一个磷酸化位点，因此通过半数位点或触发器机制发挥作用[米塞特，O.，& 罗比拉德，G. T.（1982年）《生物化学》21卷，3136 - 3142页；霍温，T.，滕霍夫 - 杜尔肯斯，R.，& 罗比拉德，G. T.（1984年）《生物化学》23卷，4335 - 4340页]。在本文中，通过使用多种不同方法确定了酶I每个二聚体有两个磷酸化位点。在一些实验中，每个二聚体发现的位点少于两个，但同时也发现酶I活性有所损失。酶I中的磷酸化残基显示出具有N3 - 磷酸组氨酰残基预期的性质。
