Chen Jian, Wang Runjie, Lu Enci, Song Shan'ai, Zhu Yingwei
Department of Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Yantai 264000, Shandong, China.
Department of Oncology, Wuxi People's Hospital Affiliated to Nanjing Medical University Wuxi 214023, Jiangsu, China.
Am J Transl Res. 2022 Feb 15;14(2):863-875. eCollection 2022.
Long-chain non-coding RNAs (lncRNAs) belong to the family of non-coding RNAs and contain more than 200 nucleotides. They are involved in the growth, apoptosis, and glycolysis of carcinoma cells. A newly discovered lncRNA, LINC00630, has been reported in colon carcinoma. In this study, we found that the expression of LINC00630 was remarkably upregulated in colon carcinoma tissues and cell lines compared with that in adjacent tissues and the NCM-460 cell lines. Knocking out LINC00630 resulted in inhibition of proliferation and glycolysis but increase in apoptosis. In addition, we confirmed the direct interaction between LINC00630 and miR-409-3p in colon carcinoma cells using bioinformatics methods and dual luciferase reporter gene assay. Finally, we demonstrated that LINC00630 could promote cell growth and glycolysis and inhibit apoptosis by functioning as a miR-409-3p sponge, and further regulate hexokinase 2 (HK2) in colon carcinoma cells. Our results confirmed that LINC00630 regulates proliferation, glycolysis, and apoptosis mainly through targeting the miR-409-3p/HK2 axis, which may explain the progression of colon carcinoma and provide a potential target for the treatment of colon carcinoma.
长链非编码RNA（lncRNAs）属于非编码RNA家族，包含超过200个核苷酸。它们参与癌细胞的生长、凋亡和糖酵解过程。一种新发现的lncRNA，即LINC00630，已在结肠癌中被报道。在本研究中，我们发现与相邻组织和NCM - 460细胞系相比，LINC00630在结肠癌组织和细胞系中的表达显著上调。敲除LINC00630导致增殖和糖酵解受到抑制，但凋亡增加。此外，我们使用生物信息学方法和双荧光素酶报告基因检测证实了结肠癌细胞中LINC00630与miR - 409 - 3p之间的直接相互作用。最后，我们证明LINC00630可以作为miR - 409 - 3p的海绵发挥作用，促进细胞生长和糖酵解并抑制凋亡，并进一步调节结肠癌细胞中的己糖激酶2（HK2）。我们的结果证实LINC00630主要通过靶向miR - 409 - 3p/HK2轴来调节增殖、糖酵解和凋亡，这可能解释了结肠癌的进展，并为结肠癌的治疗提供了一个潜在靶点。
