circITCH 通过 miR-660/TFCP2 通路抑制黑色素瘤中的细胞增殖和转移。
circITCH suppresses cell proliferation and metastasis through miR-660/TFCP2 pathway in melanoma.
机构信息
Department of Plastic surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China.
Department of Gynaecology and Obstetrics, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China.
出版信息
Cancer Med. 2022 Jun;11(12):2405-2413. doi: 10.1002/cam4.4627. Epub 2022 Mar 10.
BACKGROUND
Melanoma is an aggressive disease that is rising in incidence. Advanced melanoma is still a life-threatening disease. CircRNAs are documented to be involved in melanoma progression. But circITCH role in melanoma remains unclear.
METHODS AND RESULTS
To explore the functions of circITCH in melanoma, levels of circITCH in melanoma tissues and paracarcinoma normal tissues were detected. To study the roles of circITCH in melanoma in terms of cell proliferation and migration, in vitro and in vivo experiments were performed. Mechanism study was designed to investigate the potential regulatory effect of circITCH in melanoma. Results revealed that circITCH expression was repressed in melanoma versus adjacent normal tissues. Function study showed that circITCH suppressed melanoma cell proliferation and metastasis. The mechanism study showed that circITCH-sponged miR-660 to upregulate TFCP2 and suppress melanoma progression.
CONCLUSIONS
The circITCH/miR-660/TFCP2 axis is involved in melanoma progression hence circITCH can be a diagnostic biomarker as well as a target for treating melanoma.
背景
黑色素瘤是一种发病率不断上升的侵袭性疾病。晚期黑色素瘤仍然是一种危及生命的疾病。CircRNAs 被证明参与了黑色素瘤的进展。但 circITCH 在黑色素瘤中的作用尚不清楚。
方法和结果
为了探索 circITCH 在黑色素瘤中的功能，检测了黑色素瘤组织和癌旁正常组织中的 circITCH 水平。为了研究 circITCH 在黑色素瘤中的增殖和迁移作用，进行了体外和体内实验。设计了机制研究来探讨 circITCH 在黑色素瘤中的潜在调节作用。结果表明，circITCH 在黑色素瘤中相对于相邻的正常组织受到抑制。功能研究表明，circITCH 抑制黑色素瘤细胞的增殖和转移。机制研究表明，circITCH 海绵吸附 miR-660 以上调 TFCP2 并抑制黑色素瘤进展。
结论
circITCH/miR-660/TFCP2 轴参与了黑色素瘤的进展，因此 circITCH 可以作为诊断生物标志物以及治疗黑色素瘤的靶点。
Zotero 插件