Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China.
Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China.
Sci Total Environ. 2022 Jul 1;828:154415. doi: 10.1016/j.scitotenv.2022.154415. Epub 2022 Mar 9.
A novel microbial consortium ZY1 capable of degrading tricresyl phosphates (TCPs) was isolated, it could quickly degrade 100% of 1 mg/L tri-o-cresyl phosphate (ToCP), tri-p-cresyl phosphate (TpCP) and tri-m-cresyl phosphate (TmCP) within 36, 24 and 12 h separately and intracellular enzymes occupied the dominated role in TCPs biodegradation. Additionally, triphenyl phosphate (TPHP), 2-ethylhexyl diphenyl phosphate (EHDPP), bisphenol-A bis (diphenyl phosphate) (BDP), tris (2-chloroethyl) phosphate (TCEP) and tris (1-chloro-2-propyl) phosphate (TCPP) could also be degraded by ZY1 and the aryl-phosphates was easier to be degraded. The TCPs reduction observed in freshwater and seawater indicated that high salinity might weak the degradability of ZY1. The detected degradation products suggested that TCPs was mainly metabolized though the hydrolysis and hydroxylation. Sequencing analysis presented that the degradation of TCPs relied on the cooperation between sphingobacterium, variovorax and flavobacterium. The cytochrome P450/NADPH-cytochrome P450 reductase and phosphatase were speculated might involve in TCPs degradation. Finally, toxicity evaluation study found that the toxicity of the diesters products was lower than their parent compound based on the generation of the intracellular reactive oxygen (ROS) and the apoptosis rate of A549 cell. Taken together, this research provided a new insight for the bioremediation of TCPs in actual environment.
一种新型的能够降解磷酸三邻甲苯酯 (TCPs) 的微生物联合体 ZY1 被分离出来，它能够在 36、24 和 12 h 内分别快速降解 1 mg/L 的邻位三氯甲苯磷酸酯 (ToCP)、对位三氯甲苯磷酸酯 (TpCP) 和间位三氯甲苯磷酸酯 (TmCP)，并且细胞内酶在 TCPs 生物降解中占据主导地位。此外，ZY1 还可以降解磷酸三苯酯 (TPHP)、2-乙基己基二苯基磷酸酯 (EHDPP)、双酚 A 双 (二苯基磷酸酯) (BDP)、三 (2-氯乙基) 磷酸酯 (TCEP) 和三 (1-氯-2-丙基) 磷酸酯 (TCPP)，并且芳基磷酸酯更容易被降解。在淡水和海水中观察到的 TCPs 减少表明，高盐度可能会削弱 ZY1 的降解能力。检测到的降解产物表明，TCPs 主要通过水解和羟化作用进行代谢。序列分析表明，TCPs 的降解依赖于鞘氨醇杆菌、鞘氨醇单胞菌和黄杆菌之间的合作。推测细胞色素 P450/NADPH-细胞色素 P450 还原酶和磷酸酶可能参与 TCPs 降解。最后，毒性评估研究发现，根据细胞内活性氧 (ROS) 的产生和 A549 细胞的凋亡率，二酯产物的毒性低于其母体化合物。综上所述，这项研究为实际环境中 TCPs 的生物修复提供了新的见解。
