Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China; Biotechnology Research Department, Department of Research and Innovation, Kyaukse, Myanmar.
J Hazard Mater. 2021 Jun 15;412:125238. doi: 10.1016/j.jhazmat.2021.125238. Epub 2021 Jan 26.
In this study, the biodegradation towards aryl organophosphate flame retardants (aryl-OPFRs) was investigated by the Rhodococcus-Sphingopyxis consortium, mixture of strain Rhodococcus sp. YC-JH2 and Sphingopyxis sp. YC-JH3. The optimal ratio between the two composition strains was determined as 1:1. Under the optimum condition (pH 8, 35 °C and 0% salinity), the consortium could utilize aryl-OPFRs as sole carbon source and degrade them rapidly with half-life of 4.53, 21.11 and 23.0 h for triphenyl phosphate (TPhP), tricresyl phosphate (TCrP) and 2-ethylhexyl diphenyl phosphate (EHDPP) respectively. The consortium maintained high degrading efficiency under a wide of range of pH (6-10), temperature (20-40 °C) and salinity (0-6%). Besides, the consortium could rapidly degrade high concentration of TPhP and no inhibitory effect towards degradation speed was observed up to 500 mg/L. The effect of metal ions and surfactants was estimated. Most metal ions exhibited significant inhibition, except Zn and Pb, which showed no effect or slight promotion. Ionic surfactants could severely reduce the degrading capacity, while nonionic surfactants showed no effect. With abundant inoculation of the consortium, mineralization higher than 75% could be achieved within a week. This study provides efficient microorganisms for bioremediation of aryl-OPFRs contamination.
在这项研究中，通过 Rhodococcus-Sphingopyxis 联合体（混合菌株 Rhodococcus sp. YC-JH2 和 Sphingopyxis sp. YC-JH3）研究了芳基有机磷阻燃剂（aryl-OPFRs）的生物降解性。确定了两种组成菌株的最佳比例为 1:1。在最佳条件（pH8、35°C 和 0%盐度）下，该联合体可以将芳基-OPFRs 作为唯一碳源利用，并迅速降解，半衰期分别为 4.53、21.11 和 23.0 h，用于三苯基磷酸酯（TPhP）、三（2-氯乙基）磷酸酯（TCrP）和 2-乙基己基二苯基磷酸酯（EHDPP）。该联合体在广泛的 pH（6-10）、温度（20-40°C）和盐度（0-6%）范围内保持着较高的降解效率。此外，该联合体可以迅速降解高浓度的 TPhP，在 500 mg/L 以下的浓度范围内，降解速度没有受到抑制。还评估了金属离子和表面活性剂的影响。大多数金属离子表现出显著的抑制作用，除了 Zn 和 Pb，它们没有影响或轻微促进作用。离子表面活性剂会严重降低降解能力，而非离子表面活性剂则没有影响。通过大量接种联合体，可以在一周内实现高于 75%的矿化。这项研究为芳基-OPFRs 污染的生物修复提供了有效的微生物。
