扩增的CD4+CXCR5-PD-1+外周辅助性T样细胞及其在IgG4相关疾病中的临床意义
Expanded CD4+CXCR5-PD-1+ peripheral T helper like cells and clinical significance in IgG4-related disease.
作者信息
Zhang Panpan, Wang Mu, Chen Yu, Li Jieqiong, Liu Zheng, Lu Hui, Fei Yunyun, Feng Ruie, Zhao Yan, Zeng Xiaofeng, Zhang Wen
机构信息
Department of Rheumatology and Clinical Immunology, Chinese Academy of Medical Sciences & Peking Union Medical College; National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Ministry of Science & Technology; State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital (PUMCH); Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing 100730, China; Department of Rheumatology and Immunology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Stomatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.
出版信息
Clin Immunol. 2022 Apr;237:108975. doi: 10.1016/j.clim.2022.108975. Epub 2022 Mar 8.
To identify the phenotypic features and clinical significance of peripheral T helper (Tph) like cells in IgG4-RD, 54 untreated IgG4-RD patients and 57 healthy controls (HCs) were enrolled. Flow cytometry analysis, ELISA and correlation analysis were performed. Results indicated that percentages of CD4 + CXCR5-PD-1+ Tph like cells in the peripheral blood of IgG4-RD patients were significantly higher than those of HCs (2.27% ± 1.99% vs 1.12% ± 0.98%, P < 0.001). Expression of CD38, CD25, and TIGIT was higher, whereas that of CCR7, CD127 was lower in the Tph like cells from the IgG4-RD patients than in those from the HCs. The IgG4-RD patients with affected internal organs had higher circulating Tph like cell levels than those without (2.69% ±1.99% vs 1.23% ± 0.93%, respectively, P = 0.003). In addition, Tph like cells correlated with serum IgG and IgG4 and peripheral plasmablast levels which could be a promising biomarker for disease activity monitoring.
为了确定IgG4相关性疾病（IgG4-RD）中外周辅助性T（Tph）样细胞的表型特征及临床意义，纳入了54例未经治疗的IgG4-RD患者和57例健康对照者（HCs）。进行了流式细胞术分析、酶联免疫吸附测定（ELISA）及相关性分析。结果显示，IgG4-RD患者外周血中CD4 + CXCR5-PD-1 + Tph样细胞的百分比显著高于健康对照者（2.27% ± 1.99% 对1.12% ± 0.98%，P < 0.001）。与健康对照者相比，IgG4-RD患者的Tph样细胞中CD38、CD25和TIGIT的表达较高，而CCR7、CD127的表达较低。有内脏受累的IgG4-RD患者的循环Tph样细胞水平高于无内脏受累者（分别为2.69% ± 1.99% 对1.23% ± 0.93%，P = 0.003）。此外，Tph样细胞与血清IgG、IgG4及外周浆母细胞水平相关，这可能是监测疾病活动的一个有前景的生物标志物。
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