In situ Raman spectroscopy for real time detection of cysteine.

Cysteine serves a wide range of important biological and chemical functions and may have an association to neurodegenerative disease and cancer. Rapid, accurate analytical methods for cysteine detection are thus highly desirable. In this work, we report an investigation into the utility of in situ Raman spectroscopy as a Process Analytical Technology (PAT) for real time monitoring of cysteine. Cysteine concentrations are tracked in real time using Raman spectroscopy across a range of pharmaceutically-relevant concentrations, demonstrating the capability of Raman spectroscopy detection for in situ cysteine monitoring. The concentration range over which this analytical methodology can be applied is successfully established. As such, the results herein serve as a proof-of-principle investigation to demonstrate and evaluate the capabilities of a real time Raman spectroscopic approach for in situ cysteine detection, thus informing the range of important chemical and biological processes to which this approach can be applied. To the best of our knowledge, this is the first report of in situ Raman spectroscopy for real time monitoring of dynamically changing cysteine process concentrations.