Villemain F, Jonker M, Bach J F, Chatenoud L
Eur J Immunol. 1986 Aug;16(8):945-9. doi: 10.1002/eji.1830160812.
The immune response of 23 rhesus monkeys against different murine monoclonal antibodies (mAb) administered in vivo as immunosuppressive agents has been analyzed. Seven mAb specific for either helper-inducer (CD4 molecules) or cytotoxic/suppressor (CD8 molecules) T cells, that cross-react with monkey lymphocytes, were administered i.v. for 10 consecutive days in rhesus monkeys. Nineteen of the animals were recipients of a skin or renal allotransplant. Nineteen out of the 23 monkeys developed a significant immune response against the injected monoclonal. This response was restricted in its specificity since unrelated murine monoclonals were not recognized by the monkeys' anti-monoclonal immunoglobulins. Fine analysis of the monkeys' sera revealed that the antibodies produced against the xenogeneic proteins selectively exhibited two major specificities i.e., anti-isotypic and anti-idiotypic. On a practical basis, these results suggest that an animal already immunized against a given mAb should still be sensitive to the therapeutic effect of another monoclonal sharing the same specificity but different idiotype.
对23只恒河猴针对作为免疫抑制剂在体内施用的不同鼠单克隆抗体（mAb）的免疫反应进行了分析。给7种与猴淋巴细胞发生交叉反应、针对辅助诱导性（CD4分子）或细胞毒性/抑制性（CD8分子）T细胞的单克隆抗体静脉内连续10天施用于恒河猴。其中19只动物接受了皮肤或肾脏同种异体移植。23只猴子中有19只对注射的单克隆抗体产生了显著的免疫反应。由于无关的鼠单克隆抗体未被猴子的抗单克隆免疫球蛋白识别，这种反应在特异性上受到限制。对猴子血清的精细分析表明，针对异种蛋白产生的抗体选择性地表现出两种主要特异性，即抗同种型和抗独特型。从实际角度来看，这些结果表明，已经针对给定单克隆抗体免疫的动物对于具有相同特异性但不同独特型的另一种单克隆抗体的治疗效果仍应敏感。
