Saka Kanju, Kudo Keiko, Namera Akira, Fujii Yusuke, Noritake Kanako, Torimitsu Suguru, Makino Yohsuke, Iwase Hirotaro
Department of Forensic Medicine, Graduate School of Medicine, the University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Fukuoka University Research Institute for Toxicological Detection and Monitoring, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Sci Justice. 2022 Mar;62(2):193-202. doi: 10.1016/j.scijus.2022.02.001. Epub 2022 Feb 8.
Cyanide is a powerful and rapidly acting poison. In Japan, cyanide poisoning is rare, and regular cyanide testing can be costly and time consuming. In contrast, alcohol analysis is routinely performed in most forensic laboratories. In this study, we attempted to develop a method for the simultaneous quantification of cyanide and alcohols in blood using headspace gas chromatography (HS-GC). As nitrogen-phosphorus detection (NPD) is more sensitive to hydrogen cyanide than mass spectrometry (MS), a Deans switch was used to switch the detectors during a single run. The separation provided by three analytical columns, PoraBOND Q, CP-Sil 5 CB, and HP-INNOWax, was investigated, and PoraBOND Q was selected. The use of HS-GC-MS/NPD with a Deans switch enabled the simple and simultaneous quantification of cyanide, ethanol, and 1-propanol. Eighteen other volatile compounds were detected in the SIM/scan mode of the MS.
氰化物是一种强效且作用迅速的毒药。在日本，氰化物中毒较为罕见，定期进行氰化物检测成本高昂且耗时。相比之下，大多数法医实验室都常规进行酒精分析。在本研究中，我们试图开发一种使用顶空气相色谱法（HS-GC）同时定量血液中氰化物和酒精的方法。由于氮磷检测（NPD）对氰化氢的灵敏度高于质谱（MS），因此在单次运行过程中使用迪恩斯切换装置来切换检测器。研究了三根分析柱PoraBOND Q、CP-Sil 5 CB和HP-INNOWax提供的分离效果，最终选择了PoraBOND Q。使用带有迪恩斯切换装置的HS-GC-MS/NPD能够简单且同时定量氰化物、乙醇和1-丙醇。在MS的选择离子监测/扫描模式下还检测到了其他18种挥发性化合物。
