Gaensslen R E, Lee H C, DeGraw S S, Carroll-Reho J
Forensic Sci Int. 1986 Jul 14;31(3):145-57. doi: 10.1016/0379-0738(86)90182-9.
The effects of absorption time, titer of antiserum or lectin in the absorption stage, and elution temperature on relative antibody or lectin yield in elutes in the absorption-elution procedure were studied in bloodstains and ammoniacal extracts of bloodstains using conventional grouping reagents. In addition, monoclonal anti-A and anti-B and affinity-purified Ulex europaeus agglutinin I (UEA I) reagents were employed for comparison in these processes. Ammoniacal extracts of bloodstains and dried bloodstains on cotton substrata behaved comparably with respect to the parameters studied. The monoclonal anti-A and anti-B and UEA I reagents studied yielded satisfactory results, comparable in some cases to conventional reagents, with respect to the parameters studied.
使用传统分组试剂，研究了吸收时间、吸收阶段抗血清或凝集素的效价以及洗脱温度对吸收 - 洗脱过程中洗脱液中相对抗体或凝集素产量的影响，研究对象为血迹以及血迹的氨提取物。此外，在这些过程中使用单克隆抗 - A和抗 - B以及亲和纯化的欧洲荆豆凝集素I（UEA I）试剂进行比较。就所研究的参数而言，血迹的氨提取物和棉基质上的干血迹表现相当。在所研究的参数方面，所研究的单克隆抗 - A和抗 - B以及UEA I试剂产生了令人满意的结果，在某些情况下与传统试剂相当。
