鼠伤寒沙门氏菌半胱氨酸或蛋氨酸营养缺陷型且对1,2,4-三唑抑制有抗性的遗传不稳定性的本质
The nature of genetic instability in auxotrophs of Salmonella typhimurium requiring cysteine or methionine and resistant to inhibition by 1,2,4-triazole.
作者信息
Kingsman A J, Smith D A
出版信息
Genetics. 1978 Jul;89(3):439-51. doi: 10.1093/genetics/89.3.439.
Abstract
We tested the hypothesis that unstable suppression of auxotrophy in triazole-resistant derivatives of Cym- mutants of Salmonella typhimurium is due to reversible insertion at the Cym- site of genetic material originating in the cysALKptsHI region. We have shown that the unstable phenotype was co-transducible with markers in the cysCDHIJ region. The suppression of the Cym phenotype was recA dependent and frequencies of segregation were affected by UV irradiation. Restored enzyme activity in suppressed strains was determined by wild-type enzyme, suggesting that the unstable regions are located in cys gene regulatory regions. These results support the hypothesis. In contradiction, we found no evidence for a deletion in the cysALKptsHI region.
摘要
我们检验了这样一个假说
鼠伤寒沙门氏菌Cym-突变体的三唑抗性衍生物中营养缺陷型的不稳定抑制是由于源自cysALKptsHI区域的遗传物质可逆插入到Cym-位点所致。我们已经表明，不稳定表型与cysCDHIJ区域中的标记共转导。Cym表型的抑制是recA依赖性的，并且分离频率受紫外线照射的影响。抑制菌株中恢复的酶活性由野生型酶决定，这表明不稳定区域位于cys基因调控区域。这些结果支持了该假说。与此相反，我们没有发现cysALKptsHI区域发生缺失的证据。
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The nature of genetic instability in auxotrophs of Salmonella typhimurium requiring cysteine or methionine and resistant to inhibition by 1,2,4-triazole.鼠伤寒沙门氏菌半胱氨酸或蛋氨酸营养缺陷型且对1,2,4-三唑抑制有抗性的遗传不稳定性的本质
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