Chinese Academy of Sciences (CAS) and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Front Immunol. 2022 Feb 23;13:819881. doi: 10.3389/fimmu.2022.819881. eCollection 2022.
The TRIpartite Motif (TRIM) proteins play key roles in cell differentiation, apoptosis, development, autophagy, and innate immunity in vertebrates. In the present study, a novel TRIM9 homolog (designated as ) specifically expressed in the lymphoid organ of shrimp was identified from the Pacific whiteleg shrimp . Its deduced amino acid sequence possesses the typical features of TRIM proteins, including a RING domain, two B-boxes, a coiled-coil domain, a FN3 domain, and a SPRY domain. The transcripts of were mainly located in the lymphoid tubules of the lymphoid organ. Knockdown of could apparently inhibit the transcriptions of some genes from white spot syndrome virus (WSSV) and reduce the viral propagation in the lymphoid organ. Overexpression of in mammalian cells could activate the promoter activity of NF-κB, and an experiment in shrimp showed that knockdown of reduced the expression of in the lymphoid organ. Yeast two-hybridization and co-immunoprecipitation (Co-IP) assays confirmed that LvTRIM9-1 could directly interact with LvIMD, a key component of the IMD pathway, through its SPRY domain. These data suggest that LvTRIM9-1 could activate the IMD pathway in shrimp interaction with LvIMD. This is the first evidence to show the regulation of a TRIM9 protein on the IMD pathway through its direct interaction with IMD, which will enrich our knowledge on the role of TRIM proteins in innate immunity of invertebrates.
TRIM 蛋白在脊椎动物的细胞分化、细胞凋亡、发育、自噬和先天免疫中发挥关键作用。本研究从凡纳滨对虾中鉴定出一种新型 TRIM9 同源物（命名为 ），该蛋白特异性表达于虾的淋巴器官。其推导的氨基酸序列具有 TRIM 蛋白的典型特征，包括一个 RING 结构域、两个 B -box 结构域、一个卷曲螺旋结构域、一个 FN3 结构域和一个 SPRY 结构域。 的转录本主要位于淋巴器官的淋巴小管中。 基因的敲低明显抑制了淋巴器官中某些来自白斑综合征病毒（WSSV）的基因的转录，并降低了病毒在淋巴器官中的增殖。 在哺乳动物细胞中的过表达可以激活 NF-κB 的启动子活性，并且在虾中的 实验表明， 的敲低降低了淋巴器官中 的表达。酵母双杂交和免疫共沉淀（Co-IP）实验证实，LvTRIM9-1 通过其 SPRY 结构域可以直接与 IMD 相互作用，IMD 是 IMD 途径的关键组成部分。这些数据表明，LvTRIM9-1 可以通过与 IMD 的直接相互作用在虾中激活 IMD 途径。这是第一个证明 TRIM9 蛋白通过与 IMD 的直接相互作用调节 IMD 途径的证据，这将丰富我们对 TRIM 蛋白在无脊椎动物先天免疫中的作用的认识。
