Department of Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Department of Gynecology, Chongqing Jiangjin District Central Hospital, Chongqing, China.
J Healthc Eng. 2022 Mar 2;2022:7052066. doi: 10.1155/2022/7052066. eCollection 2022.
The aim of this study was to examine the role of B lymphoma Moloney murine leukemia virus insertion region 1 (BMI1) gene in regulating the apoptosis, invasion, and migration of human endometrial adenocarcinoma cell line (HEC-1B) cells induced by ionizing radiation. The expression of BMI1 mRNA was detected by quantitative real-time polymerase chain reaction (qRT-PCR), and the positive expression of BMI1 was detected by immunohistochemistry (IHC) staining. HEC-1 B cells were randomly divided into three groups: control group, BMI1 overexpression group, and BMI1 inhibitor group. Cell proliferation was detected by cell counting kit-8 (CCK-8); cell migration and invasion were detected by Transwell test; cell apoptosis was detected by flow cytometry; and the expression of MMP2, MMP7, MMP9, Rock1, RhoA, P53, P21, and Bax protein was detected by the western blot. The results suggested that the expression of BMI1 mRNA and tissue positive in endometrial cancer tissues was increased significantly. After ionizing radiation, compared with the control group, the proliferation, cell migration, and invasion of HEC-1B cells were increased significantly in the BMI1 overexpression group, while the proliferation, cell migration, and invasion of HEC-1B cells were decreased significantly in BMI1 inhibitor group. The apoptosis rate of BMI1 overexpression group was decreased significantly, while the BMI1 inhibitor group was increased significantly. The levels of MMP2, MMP7, MMP9, Rock1, RhoA and p53, p21, Bax protein in BMI1 overexpression group were significantly increased, while the levels of MMP2, MMP7, MMP9, Rock1, RhoA and p53, p21, Bax protein in BMI1 inhibitor group were significantly decreased. BMI1 is highly expressed in endometrial cancer tissues, and inhibiting BMI1 expression can reduce the proliferation, migration, and invasion of HEC-1B cells after ionizing radiation and promote apoptosis, which offers new insights into the clinical radiotherapy of tumors.
本研究旨在探讨 B 淋巴瘤 Moloney 鼠白血病病毒插入区 1（BMI1）基因在调节电离辐射诱导的人子宫内膜腺癌细胞系（HEC-1B）细胞凋亡、侵袭和迁移中的作用。通过实时定量聚合酶链反应（qRT-PCR）检测 BMI1mRNA 的表达，免疫组织化学（IHC）染色检测 BMI1 的阳性表达。将 HEC-1B 细胞随机分为三组：对照组、BMI1 过表达组和 BMI1 抑制剂组。通过细胞计数试剂盒-8（CCK-8）检测细胞增殖；通过 Transwell 试验检测细胞迁移和侵袭；通过流式细胞术检测细胞凋亡；通过 Western blot 检测 MMP2、MMP7、MMP9、Rock1、RhoA、P53、P21 和 Bax 蛋白的表达。结果表明，BMI1mRNA 的表达和组织阳性在子宫内膜癌组织中显著增加。在电离辐射后，与对照组相比，BMI1 过表达组 HEC-1B 细胞的增殖、细胞迁移和侵袭明显增加，而 BMI1 抑制剂组 HEC-1B 细胞的增殖、细胞迁移和侵袭明显减少。BMI1 过表达组细胞凋亡率明显降低，而 BMI1 抑制剂组则明显升高。BMI1 过表达组中 MMP2、MMP7、MMP9、Rock1、RhoA 和 p53、p21、Bax 蛋白水平明显升高，而 BMI1 抑制剂组中 MMP2、MMP7、MMP9、Rock1、RhoA 和 p53、p21、Bax 蛋白水平明显降低。BMI1 在子宫内膜癌组织中高表达，抑制 BMI1 表达可降低电离辐射后 HEC-1B 细胞的增殖、迁移和侵袭，促进凋亡，为肿瘤的临床放疗提供了新的思路。
