Kirilovsky D, Ohad I
J Biol Chem. 1986 Sep 15;261(26):12317-23.
Phycobiliproteins obtained by dissociation of phycobilisomes were reassociated in vitro with intact thylakoids or isolated photosystems I and II preparations obtained from cyanophytes (prokaryotes) or green algae (eukaryotes) to form bound phycobilisome complexes. Energy transfer from Fremyella diplosiphon phycobiliproteins to chlorophyll a of reaction centers I and II was measured in: complexes containing intact thylakoids of the cyanophytes F. diplosiphon or Anacystis nidulans and the eukaryotic algae Euglena gracilis and mutants of Chlamydomonas reinhardtii; complexes containing isolated photosystem II particles of A. nidulans or C. reinhardtii; and complexes containing reaction center I of F. diplosiphon or C. reinhardtii. Energy transfer from phycoerythrin to chlorophyll a of photosystem II could be demonstrated in complexes containing phycobilisomes bound to cyanophyte thylakoids or isolated photosystem II particles of A. nidulans or C. reinhardtii. Bound phycobilisomes did not transfer energy to photosystem II within green algae thylakoids containing altered forms of light-harvesting chlorophyll a/b-protein complex (LHC) II antenna, reduced amounts of LHC II, or chlorophyll b, or chlorophyll b-less mutants, nor to chlorophyll a of photosystem I of intact thylakoids or isolated reaction centers. We conclude that phycobilisomes can form a specific and functional association with photosystem II particles of both cyanophytes and eukaryotic thylakoids. This interaction appears to be hindered by the presence of LHC II antenna in the eukaryotic thylakoids.
通过解离藻胆体获得的藻胆蛋白在体外与完整的类囊体或从蓝藻（原核生物）或绿藻（真核生物）获得的分离的光系统I和II制剂重新结合，形成结合的藻胆体复合物。在以下体系中测量了从双歧弗氏藻藻胆蛋白到反应中心I和II的叶绿素a的能量转移：含有蓝藻双歧弗氏藻或巢状念珠藻以及真核藻类纤细裸藻和莱茵衣藻突变体的完整类囊体的复合物；含有巢状念珠藻或莱茵衣藻分离的光系统II颗粒的复合物；以及含有双歧弗氏藻或莱茵衣藻反应中心I的复合物。在含有与蓝藻类囊体结合的藻胆体或巢状念珠藻或莱茵衣藻分离的光系统II颗粒的复合物中，可以证明藻红蛋白到光系统II的叶绿素a的能量转移。结合的藻胆体不会将能量转移到含有改变形式的捕光叶绿素a/b蛋白复合物（LHC）II天线、LHC II含量减少或叶绿素b或无叶绿素b突变体的绿藻类囊体中的光系统II，也不会转移到完整类囊体或分离反应中心的光系统I的叶绿素a。我们得出结论，藻胆体可以与蓝藻和真核类囊体的光系统II颗粒形成特定的功能关联。这种相互作用似乎受到真核类囊体中LHC II天线的存在的阻碍。
