Schnitzler Lukas G, Paeger Anne, Brugger Manuel S, Schneider Matthias F, Westerhausen Christoph
Medical and Biological Physics, Technical University Dortmund, 44227 Dortmund, Germany.
Biomicrofluidics. 2022 Mar 3;16(2):024102. doi: 10.1063/5.0084084. eCollection 2022 Mar.
Single cell measurements with living specimen like, for example, the ciliated protozoan can be a challenging task. We present here a microfluidic trapping mechanism for measurements with these micro-organisms that can be used, e.g., for optical measurements to correlate cellular functions with the phase state of the lipid membrane. Here, we reversibly trap single cells in small compartments. Furthermore, we track and analyze the swimming behavior of single cells over several minutes. Before and after reversible trapping the swimming speed is comparable, suggesting that trapping does not have a large effect on cell behavior. Last, we demonstrate the feasibility of membrane order measurements on living cells using the fluorescent dye 6-lauryl-2-dimethylaminonaphthalene (Laurdan).
对活的标本（如纤毛原生动物）进行单细胞测量可能是一项具有挑战性的任务。我们在此展示一种用于对这些微生物进行测量的微流体捕获机制，该机制可用于例如光学测量，以将细胞功能与脂质膜的相态相关联。在这里，我们将单细胞可逆地捕获在小隔室中。此外，我们在几分钟内跟踪并分析单细胞的游动行为。在可逆捕获前后，游动速度相当，这表明捕获对细胞行为没有很大影响。最后，我们证明了使用荧光染料6 - 十二烷基 - 2 - 二甲基氨基萘（Laurdan）对活细胞进行膜有序测量的可行性。
