Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, France.
Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire Jean Perrin (LJP), F-75005, Paris, France.
J Exp Biol. 2020 Jun 17;223(Pt 12):jeb219253. doi: 10.1242/jeb.219253.
We present a simple device to mechanically immobilize motile cells such as ciliates. It can be used in particular for intracellular electrophysiology and microinjection. A transparent filter with holes smaller than the specimen is stretched over an outlet. A flow is induced by either a peristaltic pump or a depressurized tank, mechanically entraining cells to the bottom, where they are immobilized against the filter. The cells start swimming again as soon as the flow is stopped. We demonstrate the device by recording action potentials in and injecting a fluorescent dye into the cytosol.
我们介绍了一种简单的设备，用于机械固定运动细胞，如纤毛虫。它特别适用于细胞内电生理学和微注射。一个带有小孔的透明滤器，其孔径小于标本，被拉伸在出口上。通过蠕动泵或减压罐来产生流动，将细胞机械地卷入底部，在那里它们被固定在滤器上。一旦停止流动，细胞就会重新开始游动。我们通过记录动作电位和将荧光染料注入细胞质来演示该设备。
