通过免疫印迹电泳检测双链RNA。

Detection of double-stranded RNA by immunoblot electrophoresis.

作者信息

Gabriel C J

出版信息

J Virol Methods. 1986 Jul;13(4):279-83. doi: 10.1016/0166-0934(86)90052-2.

Abstract

A procedure is described which permits the serological detection of double-stranded RNA (dsRNA) which has been electrophoretically transferred from agarose gels to Zeta-Probe blotting membranes. Cucumber mosaic virus replicative forms of RNAs 1-3 and cassava (Manihot esculenta) clone 'Secundina' dsRNAs can be routinely detected from 1 g of leaf tissue. This procedure allows for the detection and size estimation of dsRNAs in crude nucleic acid preparations.

摘要

本文描述了一种方法，该方法可用于血清学检测从琼脂糖凝胶电泳转移至Zeta-Probe印迹膜上的双链RNA（dsRNA）。从1克叶片组织中可常规检测到黄瓜花叶病毒RNA 1-3的复制形式以及木薯（Manihot esculenta）克隆“Secundina”的dsRNA。该方法可用于检测粗核酸制剂中的dsRNA并估计其大小。