Anatomic variations in mesiobuccal root (MBR) of maxillary molars are common. This variation is found to be more common in maxillary first molars as compared to second molars. However, finding three independent mesiobuccal (MB) canals in the MBR of maxillary molars is clinically a rare entity. With the use of magnification, illumination, and cone-beam computed tomography, combined with the skill of the operator, there is an increased possibility of detecting such additional canals. The present case report describes the successful clinical management of a second molar in a 58-year-old female patient having three MBR canals (MB1, MB2, and MB3) with a Vertucci's Type VIII canal configuration and an almost obliterated pulp chamber. The canals were prepared using hand and rotary instruments, followed by obturation. Very few such cases have been documented clinically in the literature.