Räsänen L, Lehto M, Reunala T, Leinikki P
J Invest Dermatol. 1986 Jan;86(1):9-12. doi: 10.1111/1523-1747.ep12283693.
The present investigation introduces a method for purification of human epidermal Langerhans cells (LC). The method is based on the attachment of LC to IgG-coated sheep erythrocyte monolayers via their Fc receptors. To optimize the enrichment assay, several variables were tested. The best results were obtained when epidermal cells were centrifuged against erythrocyte monolayers; the purification procedure was performed at 4 degrees C in the presence of 5% fetal calf serum, using about 6 X 10(6) epidermal cells per erythrocyte plate (diameter 5 cm). The average purity of the recovered LC was 80.9% and LC-depleted fractions contained an average of 0.5% DR-positive cells. LC were able to enhance significantly leukoagglutinin- and purified protein derivative-induced T lymphocyte proliferation and leukocyte migration inhibitory factor production.
本研究介绍了一种纯化人表皮朗格汉斯细胞（LC）的方法。该方法基于LC通过其Fc受体与包被IgG的绵羊红细胞单层结合。为优化富集试验，对几个变量进行了测试。当表皮细胞与红细胞单层进行离心时获得了最佳结果；纯化过程在4℃、5%胎牛血清存在的条件下进行，每个红细胞平板（直径5 cm）使用约6×10⁶个表皮细胞。回收的LC的平均纯度为80.9%，不含LC的组分平均含有0.5%的DR阳性细胞。LC能够显著增强白细胞凝集素和纯化蛋白衍生物诱导的T淋巴细胞增殖以及白细胞迁移抑制因子的产生。
