Sun Lin, Peng Bo, Zhou Jun, Wang Ping, Mo Yuqing, Xu Guofang, Tao Yi, Song Hejie, Tang Wei, Jin Meiling
Department of Respiratory and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Institute of Respiratory Diseases, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
J Asthma Allergy. 2022 Mar 6;15:315-326. doi: 10.2147/JAA.S345759. eCollection 2022.
Allergic asthma is a heterogeneous disease with complex underlying mechanisms. Cytokines are key mediators in immune system and potential indicators of disease status. The aim of this study is to compare the difference of serum cytokine profile in allergic asthma patients with different disease severity and explore candidate biomarkers for disease monitoring and targeting therapeutic agents.
A total of 40 allergic asthmatics (mild, n=22; moderate-to-severe, n=18) were included in this study. Serum samples, lung function and exhaled nitric oxide data were collected from each subject. A Meso Scale Discovery (MSD) electrochemiluminescence platform was applied to access serum levels of 33 cytokines. Serum cytokine profile was compared between mild and moderate-to-severe allergic asthmatics, and the correlation between serum cytokine levels, lung function and exhaled nitric oxide were analyzed.
Moderate-to-severe allergic asthmatics displayed higher levels of eotaxin-1, eotaxin-2, MCP-1, MCP-2, MCP-3, YKL-40 and lower IL-23, IL-31 and TRAIL in serum in comparison with mild allergic asthmatics. Serum YKL-40, eotaxin-1 and MCP-1 had the best ability to discriminate mild and moderate-to-severe allergic asthmatics, with an AUC of 0.833, 0.811 and 0.760. Serum IP-10 was positively correlated with FeNO levels, while FnNO displayed a strong positive correlation with serum IL-25.
Compared with mild allergic asthmatics, significant increase in serum eotaxin-1, eotaxin-2, MCP-1, MCP-2, MCP-3, YKL-40 and decrease in serum IL-23, IL-31 and TRAIL was noted in moderate-to-severe allergic asthmatics. YKL-40, eotaxin-1 and MCP-1 might be candidate biomarkers in reflecting severity in allergic asthma patients.
过敏性哮喘是一种具有复杂潜在机制的异质性疾病。细胞因子是免疫系统中的关键介质，也是疾病状态的潜在指标。本研究旨在比较不同疾病严重程度的过敏性哮喘患者血清细胞因子谱的差异，并探索用于疾病监测和靶向治疗药物的候选生物标志物。
本研究共纳入40例过敏性哮喘患者（轻度，n = 22；中度至重度，n = 18）。收集每位受试者的血清样本、肺功能和呼出一氧化氮数据。应用Meso Scale Discovery（MSD）电化学发光平台检测33种细胞因子的血清水平。比较轻度和中度至重度过敏性哮喘患者的血清细胞因子谱，并分析血清细胞因子水平、肺功能和呼出一氧化氮之间的相关性。
与轻度过敏性哮喘患者相比，中度至重度过敏性哮喘患者血清中嗜酸性粒细胞趋化因子-1、嗜酸性粒细胞趋化因子-2、单核细胞趋化蛋白-1、单核细胞趋化蛋白-2、单核细胞趋化蛋白-3、YKL-40水平较高，而白细胞介素-23、白细胞介素-31和肿瘤坏死因子相关凋亡诱导配体水平较低。血清YKL-40、嗜酸性粒细胞趋化因子-1和单核细胞趋化蛋白-1区分轻度和中度至重度过敏性哮喘患者的能力最强，曲线下面积分别为0.833、0.811和0.760。血清干扰素γ诱导蛋白10与呼出一氧化氮水平呈正相关，而呼出一氧化氮与血清白细胞介素-25呈强正相关。
与轻度过敏性哮喘患者相比，中度至重度过敏性哮喘患者血清中嗜酸性粒细胞趋化因子-1、嗜酸性粒细胞趋化因子-2、单核细胞趋化蛋白-1、单核细胞趋化蛋白-2、单核细胞趋化蛋白-3、YKL-40显著升高，血清白细胞介素-23、白细胞介素-31和肿瘤坏死因子相关凋亡诱导配体降低。YKL-40、嗜酸性粒细胞趋化因子-1和单核细胞趋化蛋白-1可能是反映过敏性哮喘患者病情严重程度的候选生物标志物。
