Fowden A L, Mao X Z, Comline R S
J Endocrinol. 1986 Aug;110(2):225-31. doi: 10.1677/joe.0.1100225.
The effects of fetal pancreatectomy on the growth and metabolism of the fetal sheep were investigated in chronically catheterized animals during the last third of gestation. Fetal pancreatectomy reduced body weight and crown-rump length at delivery near term (term 145 days). Body weight was affected more than body length so the ratio of weight to length was significantly less after pancreatectomy than in intact animals (P less than 0.05). Pancreatectomized fetuses appeared to maintain a normal growth rate for 5-10 days after surgery but thereafter showed no further significant increase in body weight. When all the data from the intact and pancreatectomized fetuses were combined, there was a significant positive correlation between the plasma insulin concentration in utero and the body weight at delivery near term. The majority of organs studied were reduced in absolute weight after pancreatectomy but only the spleen and thymus were proportionally lighter when the weights were expressed as a percentage of body weight. Brain and placental weights were similar in intact and pancreatectomized fetuses. Over the range of values observed in utero, there were significant inverse correlations between the log plasma insulin level and the mean plasma concentrations of glucose, lactate, fructose and alpha-amino-nitrogen in individual intact and pancreatectomized fetuses. Insulin infusion into pancreatectomized fetuses restored the metabolite concentrations to their normal values within 48 h of infusion. The results demonstrate that insulin has a vital role in regulating fetal growth and metabolism in utero.
在妊娠晚期，对长期插管的动物进行了胎儿胰腺切除术，以研究其对胎羊生长和代谢的影响。近足月分娩时（足月为145天），胎儿胰腺切除术降低了出生时的体重和顶臀长度。体重受影响比体长更明显，因此胰腺切除术后体重与体长的比值显著低于未手术的动物（P<0.05）。胰腺切除的胎儿在手术后5-10天似乎维持正常生长速率，但此后体重未进一步显著增加。当将未手术和胰腺切除胎儿的所有数据合并时，子宫内血浆胰岛素浓度与近足月分娩时的体重之间存在显著正相关。胰腺切除术后，大多数研究器官的绝对重量减轻，但仅脾脏和胸腺在以体重百分比表示时相对较轻。未手术和胰腺切除胎儿的脑重和胎盘重量相似。在子宫内观察到的值范围内，未手术和胰腺切除的单个胎儿中，血浆胰岛素水平的对数与葡萄糖、乳酸、果糖和α-氨基氮的平均血浆浓度之间存在显著负相关。向胰腺切除的胎儿输注胰岛素在输注后48小时内将代谢物浓度恢复到正常水平。结果表明，胰岛素在调节子宫内胎儿生长和代谢方面起着至关重要的作用。
