Genetic Diversity of Isolates from Patients with Gastric Diseases in Isfahan.

BACKGROUND

(), a spiral-shaped bacterium colonizing the human stomach, is generally acquired in childhood. This pathogen is highly diverse and can be used as genetic markers for predict the history of human migrations. This study aimed to determine the genetic diversity of isolates from patients with dyspepsia by the multi-locus sequence typing (MLST) and update data on the prevalence of among Iranian dyspeptic patients.

MATERIALS AND METHODS

In this descriptive cross-sectional study, 165 gastric biopsy specimens were obtained from patients with dyspepsia referred to Dr. Shariati Hospital of Isfahan, Iran, from April to July 2018. The status of infection was determined by FISH in paraffin-embedded biopsy specimens. MLST of seven housekeeping genes was performed for 20 isolates. The phylogenetic tree was plotted using CLC v8 and iTol software.

RESULTS

The overall prevalence of infection was 53.3%. In the results of the analysis of MLST, a total of 14 new STs were recorded. The results of the global analysis showed that all the isolates, with a wide diversity, have a genetic affinity with members of the European population, such as Italy and Russia, and are in the hpEurope haplotype.

CONCLUSION

Given the high prevalence of infection in this region, early and accurate identification of patients seems necessary. Sequence analysis and determination of the origin of the phylogeny of strains can be effective in clinical management and monitoring of risk factors for chronic and recurrence of infection.