Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China.
Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China.
STAR Protoc. 2022 Mar 7;3(1):101219. doi: 10.1016/j.xpro.2022.101219. eCollection 2022 Mar 18.
RNA interference (RNAi) is a technique used for posttranscriptional gene silencing, but lepidopteran insects are not sensitive to RNAi. Here, we present a protocol for knocking down the expression level of target genes by RNAi in embryos. We describe the preparation of double-stranded RNAs (dsRNAs) of target genes, followed by microinjection of embryos at different developmental stages, with single or mixed dsRNA. Finally, we use RT-qPCR to verify RNAi efficiency. For complete details on the use and execution of this protocol, please refer to Xu et al. (2021).
RNA 干扰 (RNAi) 是一种用于转录后基因沉默的技术，但鳞翅目昆虫对 RNAi 不敏感。在这里，我们提供了一种在 胚胎中通过 RNAi 敲低靶基因表达水平的方案。我们描述了靶基因双链 RNA(dsRNA)的制备，然后在不同的发育阶段对胚胎进行单或混合 dsRNA 的显微注射。最后，我们使用 RT-qPCR 来验证 RNAi 效率。有关此方案使用和执行的完整详细信息，请参阅 Xu 等人。(2021)。
