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采用Ficoll梯度法进行骨髓细胞分离。

Bone marrow cell separation on Ficoll gradient.

作者信息

Sulc K, Neuwirt J, Trávnícek T, Kobylka P, Radikovská E

出版信息

Haematologia (Budap). 1977;11(1-2):41-6.

PMID:352852
Abstract

Cell fraction from rat and murine bone marrow were obtained by sedimentation at unit gravity. A linear gradient of polymerized sucrose (Ficoll) was used and some properties of individual cell fractions were studied. The 3rd cell fraction containing the lymphoblasts and lymphocytes contained also stem cells.

摘要

通过单位重力沉降法获得大鼠和小鼠骨髓的细胞组分。使用聚合蔗糖(菲可)的线性梯度,并研究了各个细胞组分的一些特性。包含淋巴母细胞和淋巴细胞的第3个细胞组分中也含有干细胞。

相似文献

1
Bone marrow cell separation on Ficoll gradient.采用Ficoll梯度法进行骨髓细胞分离。
Haematologia (Budap). 1977;11(1-2):41-6.
2
Separation of megakaryocytes from rat bone marrow cells using velocity sedimentation in an isokinetic gradient ficoll in tissue culture medium.在组织培养基中的等速梯度菲可中利用速度沉降法从大鼠骨髓细胞中分离巨核细胞。
J Cell Physiol. 1976 Jul;88(3):317-22. doi: 10.1002/jcp.1040880307.
3
[Application of sedimentation at 1 g on a Ficoll gradient to the separation of bone marrow cells].
C R Seances Soc Biol Fil. 1981;175(2):144-51.
4
Elimination of red blood cells from marrow suspension prior to transplantation using polysaccharide-ditrizoate gradient centrifugation. Experimental studies.
Folia Haematol Int Mag Klin Morphol Blutforsch. 1989;116(3-4):469-73.
5
Functional characteristics of a Ficoll-separated mouse bone marrow cell population involved in skin allograft prolongation.参与同种异体皮肤移植延长的经Ficoll分离的小鼠骨髓细胞群体的功能特性。
J Immunol. 1982 Oct;129(4):1584-8.
6
CFU-c enrichment from human bone marrow using a discontinuous Percoll gradient and soybean agglutinin in comparison with Ficoll-paque.与Ficoll-paque相比,使用不连续Percoll梯度和大豆凝集素从人骨髓中富集CFU-c
Clin Exp Immunol. 1983 Aug;53(2):491-6.
7
Purification of murine bone-marrow-derived granulocyte-macrophage colony-forming cells.小鼠骨髓来源的粒细胞-巨噬细胞集落形成细胞的纯化
Exp Hematol. 1987 Mar;15(3):243-50.
8
Density gradient separation of hematopoietic stem cells in autologous bone marrow transplantation.自体骨髓移植中造血干细胞的密度梯度分离
Haematologica. 1991 Mar;76 Suppl 1:18-21.
9
Isolation of bone marrow-derived stem cells using density-gradient separation.使用密度梯度分离法分离骨髓来源的干细胞。
Exp Hematol. 2007 Feb;35(2):335-41. doi: 10.1016/j.exphem.2006.09.014.
10
[Separation of a rat thymocyte population in a ficoll-paque gradient. II. Dynamics of the cell count in different media and the effect of thymolytic factors].
Tsitologiia. 1982 Jun;24(6):704-9.

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