[糖类抗原19-9酶免疫测定]

[CA 19-9 enzyme immunoassay].

作者信息

Ota H, Odani H, Watanabe H, Satomura Y, Takemori Y, Okai T, Sawabu N, Mai M, Hattori N, Hashimoto T

出版信息

Gan No Rinsho. 1986 Aug;32(9):993-7.

Abstract

The carbohydrate antigenic determinant (CA 19-9) EIA kit (FUJIREVIO INC) was examined both experimentally and clinically. In the basic study, the standard curve showed linearity from to 240 U/ml, and the intra-and interassay reproducibility was good. Serum levels of CA 19-9 were determined by EIA in 246 cancer patients and 180 patients with benign diseases, and CA 19-9 levels by EIA were compared with those by RIA in concurrent measurement. There was an excellent correlation between the two. The positive of CA 19-9 EIA was almost the same as that of CA 19-9 RIA in cancer patients. False-positive cases by CA 19-9 EIA were somewhat more numerous than those by CA 19-9 RIA in patients with benign diseases, especially liver diseases. However, CA 19-9 assay by EIA is simple and has an excellent reproducibility and accuracy, as does that by RIA. Thus, the CA 19-9 EIA is a very useful assay.

摘要

对碳水化合物抗原决定簇（CA 19-9）酶免疫分析试剂盒（富士瑞必欧株式会社）进行了实验和临床研究。在基础研究中，标准曲线在0至240 U/ml范围内呈线性，批内和批间重复性良好。采用酶免疫分析法测定了246例癌症患者和180例良性疾病患者的血清CA 19-9水平，并在同步检测中比较了酶免疫分析法和放射免疫分析法测定的CA 19-9水平。两者之间存在极好的相关性。在癌症患者中，CA 19-9酶免疫分析的阳性率与CA 19-9放射免疫分析的阳性率几乎相同。在良性疾病患者，尤其是肝病患者中，CA 19-9酶免疫分析的假阳性病例比CA 19-9放射免疫分析的略多。然而，酶免疫分析法检测CA 19-9与放射免疫分析法一样，操作简单，具有出色的重复性和准确性。因此，CA 19-9酶免疫分析是一种非常有用的检测方法。