Graduate School of Pharmaceutical Sciences, Tokushima University, 1-78-1 Shomachi, Tokushima, 770-8505, Japan.
Faculty of Pharmaceutical Sciences, Tokushima University, 1-78-1 Shomachi, Tokushima, 770-8505, Japan.
Anal Sci. 2022 Mar;38(3):623-626. doi: 10.1007/s44211-022-00075-w. Epub 2022 Mar 8.
The inner product (IP) of RGB unit vectors' approach for detecting color transition has further been applied to a feedback-based flow ratiometric titration, including nonaqueous titration. While the flow ratio of titrand/titrant containing an indicator was varying, the video image of the merged solution was taken with a digital microscope downstream. The indicator's color was converted to an RGB unit vector. The change in IP between vectors was used for determining the equivalence point. The concept was successfully applied to the determinations of drug and vinegar samples with reasonable throughput rate (> 18 s/titration) and precision (RSD < 4.4%).
用于检测颜色转变的 RGB 单位向量逼近内积（IP）进一步应用于基于反馈的流动比滴定法中，包括非水滴定法。在含有指示剂的滴定剂/滴定剂的流量比发生变化时，使用数字显微镜在下游拍摄混合溶液的视频图像。将指示剂的颜色转换为 RGB 单位向量。通过向量之间 IP 的变化来确定等当点。该概念成功应用于药物和醋样品的测定，具有合理的吞吐量（> 18 s/滴定）和精密度（RSD < 4.4%）。
