Research Center of Natural Resources of Chinese Medicinal Materials and Ethnic Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, China.
College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China.
Z Naturforsch C J Biosci. 2022 Mar 15;77(5-6):197-206. doi: 10.1515/znc-2021-0201. Print 2022 May 25.
The aim is to establish a model of nonalcoholic fatty liver disease (NAFLD) caused by feeding with high-fat, high-fructose, and high-cholesterol diet (HFFCD) in golden hamsters, and to investigate the characteristics of the NAFLD model and metabolite changes of liver tissue. Golden hamsters were fed HFFCD or control diets for six weeks. Body weight, abdominal fat index, and liver index was assessed, serum parameters, hepatic histology, and liver metabolites were examined. The results showed that body weight, abdominal fat, and liver index of hamsters were significantly increased in the model group, the level of serum total cholesterol (TC), triglyceride (TG), and low density lipoprotein-cholesterol (LDL-C) were significantly increased in model group as well, and high density lipoprotein-cholesterol (HDL-C) was significantly decreased. In addition, lipid deposition in liver tissue formed fat vacuoles of different sizes. Metabonomics analysis of the liver showed that the metabolic pathways of sphingolipid, glycerophospholipids, and arginine biosynthesis were disordered in the NAFLD model. The modeling method is simple, short time, and uniform. It can simulate the early fatty liver caused by common dietary factors, and provides an ideal model for the study of the initial pathogenesis and therapeutic drugs for NAFLD.
目的是建立一种通过高脂、高果糖和高胆固醇饮食（HFFCD）喂养诱导金黄地鼠非酒精性脂肪性肝病（NAFLD）的模型，并研究 NAFLD 模型的特点和肝组织代谢物的变化。金黄地鼠分别用 HFFCD 或对照饮食喂养 6 周。评估体重、腹部脂肪指数和肝指数，检测血清参数、肝组织学和肝组织代谢物。结果显示，模型组地鼠体重、腹部脂肪和肝指数显著增加，血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）水平也显著升高，高密度脂蛋白胆固醇（HDL-C）显著降低。此外，肝组织中的脂质沉积形成了不同大小的脂肪空泡。肝代谢组学分析显示，NAFLD 模型中鞘脂、甘油磷脂和精氨酸生物合成代谢途径紊乱。该建模方法简单、耗时短、结果均一。它可以模拟常见饮食因素引起的早期脂肪肝，为 NAFLD 的起始发病机制和治疗药物的研究提供了理想的模型。
