Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, Shaanxi Province, China.
Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, Shaanxi Province, China; Department of Otolaryngology, The First Hospital, Xiamen University, Xiamen, 361005, Fujian Province, China.
Gene Expr Patterns. 2022 Jun;44:119235. doi: 10.1016/j.gep.2022.119235. Epub 2022 Mar 11.
Mammalian inner ear cells are produced during embryonic development. NIN1/RPN12 binding protein 1 homolog (Nob1), a ribosome assembly factor, is believed to be involved in transcriptional regulation and may play an important role in mediating certain physiological and pathological functions.
We investigated the expression levels and distribution patterns of Nob1 in rat cochlea at different developmental stages using reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and immunofluorescence microscopy (P0, P3, P7, P14, and P30, n = 11 per age group).
The expression of Nob1 was gradually upregulated from Postnatal day (P) 0 to P14 and stabilized at P30. Our results analyzing Nob1 expression at P0 and P3 in the rat cochleae revealed expression in the greater epithelial ridge cells, lesser epithelial ridge cells, and spiral ganglion neurons (SGNs), whereas low levels of Nob1 expression were observed in the stria vascularis cells. During the development of the cochlea, the organ of Corti undergoes dramatic architectural remodeling. Nob1 expression was stably detected in supporting cells and hair cells from P7 to P30, and its expression gradually increased in the SGNs.
Thus, the present study revealed that upregulation of Nob1 might explain the maturation of the cytoarchitecture in the cochlea during the postnatal period, and a complete understanding of its function in the mammalian inner ear may help to develop strategies to resolve auditory hair cell loss.
哺乳动物内耳细胞是在胚胎发育过程中产生的。核糖体组装因子 NIN1/RPN12 结合蛋白 1 同源物（Nob1）被认为参与转录调控，可能在介导某些生理和病理功能方面发挥重要作用。
我们使用逆转录-聚合酶链反应（RT-PCR）、Western blot 分析和免疫荧光显微镜检查（P0、P3、P7、P14 和 P30，每组 11 只），研究了 Nob1 在大鼠耳蜗不同发育阶段的表达水平和分布模式。
从出生后第 0 天（P0）到 P14 天，Nob1 的表达逐渐上调，并在 P30 天稳定下来。我们在大鼠耳蜗中分析 P0 和 P3 时 Nob1 的表达结果表明，它在大上皮嵴细胞、小上皮嵴细胞和螺旋神经节神经元（SGN）中表达，而在血管纹细胞中表达水平较低。在耳蜗发育过程中，柯蒂器经历了剧烈的结构重塑。从 P7 到 P30，Nob1 表达稳定地检测到在内耳支持细胞和毛细胞中，其在 SGN 中的表达逐渐增加。
因此，本研究表明 Nob1 的上调可能解释了出生后耳蜗细胞结构的成熟过程，对其在内耳中的功能的全面了解可能有助于制定解决听觉毛细胞损失的策略。
