Department of Entomology, MOA Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China.
Department of Entomology, Kansas State University, Agricultural Research Center-Hays, Hays, KS, USA.
Pest Manag Sci. 2022 Jun;78(6):2378-2389. doi: 10.1002/ps.6868. Epub 2022 Apr 21.
High temperatures will occur more frequently with global warming, with potential impacts on the efficacy of biological control agents. Heat shock proteins (HSPs) are induced by high temperature, but their possible roles in pest resistance to entomopathogens remain unexplored. We investigated the effects of high temperature (35 °C) on Helicoverpa armigera resistance to H. armigera nucleopolyhedrovirus (HearNPV) and the putative roles of HSPs in this process.
Even short periods (24 h) of high temperature (35 °C) reduced mortality in HearNPV-infected H. armigera larvae. Sustained 35 °C exposure significantly shortened developmental time, and increased fresh weight and locomotor activity in infected larvae. Moreover, high temperature inhibited virus replication and thickened the epidermis of H. armigera, resulting in reduced spread of infection from cadavers. Real-time polymerase chain reaction (PCR) analysis showed that expression of 11 HSP genes was altered by the 35 °C treatment, and that mostly small heat shock protein (sHSP) genes were up-regulated, the same sHSPs were induced when larvae were infected with HearNPV. Finally, RNA interference (RNAi) suppression of these sHSPs showed that only Hsp24.91 and Hsp21.8 diminished H. armigera defensive responses to HearNPV infection.
Even short periods of exposure to high temperature can significantly reduce susceptibility of H. armigera larvae to HearNPV by stimulating the production of sHSPs which enhance immune responses, with important implications for the use of entomopathogens as biological control agents under global warming scenarios. © 2022 Society of Chemical Industry.
随着全球变暖，高温天气将更加频繁出现，这可能会对生物防治剂的效果产生影响。热休克蛋白（HSPs）是由高温诱导产生的，但它们在害虫对昆虫病原物抗性中的可能作用仍未得到探索。我们研究了高温（35°C）对棉铃虫对棉铃虫核多角体病毒（HearNPV）抗性的影响，以及 HSPs 在这一过程中的可能作用。
即使是短暂的高温（35°C）暴露（24 小时）也会降低感染 HearNPV 的棉铃虫幼虫的死亡率。持续的 35°C 暴露会显著缩短发育时间，并增加感染幼虫的鲜重和运动活性。此外，高温抑制了病毒的复制，并使棉铃虫的表皮变厚，从而减少了从尸体上感染的传播。实时聚合酶链反应（PCR）分析表明，11 种 HSP 基因的表达因 35°C 处理而改变，大多数小热休克蛋白（sHSP）基因上调，当幼虫感染 HearNPV 时，同样的 sHSP 被诱导。最后，这些 sHSP 的 RNA 干扰（RNAi）抑制表明，只有 Hsp24.91 和 Hsp21.8 降低了棉铃虫对 HearNPV 感染的防御反应。
即使是短暂的高温暴露也可以通过刺激 sHSPs 的产生来显著降低棉铃虫幼虫对 HearNPV 的敏感性，从而增强免疫反应，这对在全球变暖情景下使用昆虫病原物作为生物防治剂具有重要意义。© 2022 化学工业协会。
