Gillan J E, Pape K E, Cutz E
Pediatr Res. 1986 Sep;20(9):828-33. doi: 10.1203/00006450-198609000-00003.
The pulmonary neuroendocrine (NE) cells, from 16 term infants dying at 1-4 days of age from birth asphyxia, were immuno stained for bombesin-like immunoreactivity by the immunoperoxidase method. The distribution and frequency of bombesin-immunoreactive NE cells were quantified morphometrically and correlated with the presence or absence of brainstem function and persistent fetal circulation (PFC). In infants with loss of brainstem function, the frequency of bombesin immunoreactive NE cells was significantly increased compared to infants with intact brainstem function, i.e. meconium aspiration with PFC. Infants with brainstem injury, with one exception, failed to develop PFC. Pathological changes in the tegmentum of the brainstem, i.e. containing the respiratory center, correlated in nine of 10 cases with loss of brainstem function. These data suggest an inverse relationship between brainstem function, release of bombesin-like peptide from the pulmonary NE cells and the functional state of the pulmonary vasculature. Intact brainstem function appears to be essential for both the release of bombesin-like peptide from the NE cells and for pulmonary vasoconstriction leading to PFC; absence of brainstem function is, on the other hand, associated with failure to release bombesin-like peptide and loss of PFC type reactivity in the pulmonary vasculature. However, it appears unlikely that bombesin itself is a direct mediator of pulmonary vasoconstriction.
对16例出生后1 - 4天因出生窒息死亡的足月儿的肺神经内分泌（NE）细胞，采用免疫过氧化物酶法进行蛙皮素样免疫反应染色。对蛙皮素免疫反应性NE细胞的分布和频率进行形态计量学定量，并与脑干功能及持续性胎儿循环（PFC）的有无相关联。与脑干功能完整的婴儿（即胎粪吸入伴PFC）相比，脑干功能丧失的婴儿中蛙皮素免疫反应性NE细胞的频率显著增加。除1例外，脑干损伤的婴儿均未发生PFC。脑干被盖部（即包含呼吸中枢）的病理变化在10例中有9例与脑干功能丧失相关。这些数据表明脑干功能、肺NE细胞释放蛙皮素样肽与肺血管系统的功能状态之间存在负相关关系。完整的脑干功能似乎对于NE细胞释放蛙皮素样肽以及导致PFC的肺血管收缩均至关重要；另一方面，脑干功能缺失与蛙皮素样肽释放失败及肺血管系统中PFC型反应性丧失相关。然而，蛙皮素本身似乎不太可能是肺血管收缩的直接介质。
