慢性 HIV-1 感染中 HIV-1 DNA 水平低与 HLA Ⅰ类分子之间的关联。

Association between low levels of HIV-1 DNA and HLA class I molecules in chronic HIV-1 infection.

机构信息

Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Vita-Salute San Raffaele University, Milan, Italy.

出版信息

PLoS One. 2022 Mar 15;17(3):e0265348. doi: 10.1371/journal.pone.0265348. eCollection 2022.

DOI:10.1371/journal.pone.0265348

PMID:35290394

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8923435/

Abstract

BACKGROUND

HLA-B27 and -B57 were found in people with low levels of HIV-1 DNA, suggesting that HLA class I molecules may influence the size of HIV-1 reservoir. Aim of the study was to explore the association between HLA class I molecules and HIV-1 DNA in people with chronic HIV-1 infection.

METHODS

Post-hoc analysis of the APACHE trial, on adults with chronic HIV-1 infection, prolonged suppressive antiretroviral therapy and good immunological profile. HIV-1 DNA was quantified in peripheral blood mononuclear cells (PBMCs); HLA-A, -B and -C were tested on genomic DNA. Crude odds ratios (OR) with their respective 95% Wald confidence intervals (95% CIs) were estimated by univariable logistic regression for HLAs with a p-value <0.10.

RESULTS

We found 78 and 18 patients with HIV-1 DNA ≥100 copies/106PBMCs and with HIV-1 DNA <100 copies/106PBMCs, respectively. HLA-A24 was present in 21 (29.6%) participants among subjects with HIV-1 DNA ≥100 copies/106PBMCs and 1 (5.9%) among those with HIV-1 DNA <100 copies/106PBMCs (OR = 5.67, 95%CI = 0.79-46.03; p = 0.105); HLA-B39 was present in 1 (1.4%) with HIV-1 DNA ≥100 copies/106PBMCs and in 3 (17.6%) with HIV-1 DNA <100 copies/106PBMCs (OR = 13.71, 95%CI = 1.33-141.77; p = 0.028) and HLA-B55 in 3 (4.2%) and 3 (17.6%), respectively (OR = 4.43, 95%CI = 0.81-24.29; p = 0.087). All the three patients with HLA-B39 and HIV-1 DNA <100 copies/106PBMCs did not have HLA-A24.

CONCLUSIONS

In patients with HIV-1 infection who maintained a good virological and immunological profile, HLA-B39 and -B55 may be associated with lower levels of HIV-1 DNA.

摘要

背景

在 HIV-1 DNA 水平较低的人群中发现了 HLA-B27 和 -B57，这表明 HLA Ⅰ类分子可能影响 HIV-1 储存库的大小。本研究旨在探讨慢性 HIV-1 感染人群中 HLA Ⅰ类分子与 HIV-1 DNA 之间的关系。

方法

对慢性 HIV-1 感染、长期抑制性抗逆转录病毒治疗和良好免疫状况的 APACHE 试验进行了事后分析。外周血单核细胞 (PBMC) 中定量检测 HIV-1 DNA；基因组 DNA 上检测 HLA-A、-B 和 -C。通过单变量逻辑回归估计 HLA 的粗比值比 (OR) 及其各自 95% Wald 置信区间 (95%CI)，对于 p 值<0.10 的 HLA。

结果

我们发现分别有 78 例和 18 例 HIV-1 DNA≥100 拷贝/106PBMC 和 HIV-1 DNA<100 拷贝/106PBMC 的患者。在 HIV-1 DNA≥100 拷贝/106PBMC 的患者中，有 21 例（29.6%）存在 HLA-A24，而在 HIV-1 DNA<100 拷贝/106PBMC 的患者中，仅 1 例（5.9%）存在 HLA-A24（OR=5.67，95%CI=0.79-46.03；p=0.105）；在 HIV-1 DNA≥100 拷贝/106PBMC 的患者中有 1 例（1.4%）存在 HLA-B39，在 HIV-1 DNA<100 拷贝/106PBMC 的患者中有 3 例（17.6%）存在 HLA-B39（OR=13.71，95%CI=1.33-141.77；p=0.028），在 HIV-1 DNA≥100 拷贝/106PBMC 的患者中有 3 例（4.2%）存在 HLA-B55，在 HIV-1 DNA<100 拷贝/106PBMC 的患者中有 3 例（17.6%）存在 HLA-B55（OR=4.43，95%CI=0.81-24.29；p=0.087）。所有 3 例 HLA-B39 且 HIV-1 DNA<100 拷贝/106PBMC 的患者均未检出 HLA-A24。