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豌豆中关键植物化学物质的鉴定与定量——将化合物与感官特性联系起来。

Identification and quantification of key phytochemicals in peas - Linking compounds with sensory attributes.

作者信息

Cosson A, Meudec E, Ginies C, Danel A, Lieben P, Descamps N, Cheynier V, Saint-Eve A, Souchon I

机构信息

Univ Paris Saclay, UMR SayFood, AgroParisTech, INRAE, F-78850 Thiverval Grignon, France; Roquette Frères, 10 rue haute loge, F-62136 Lestrem, France.

SPO, INRAE, Univ Montpellier, Institut Agro Montpellier Supagro, Montpellier, France; INRAE, PROBE Research Infrastructure, Polyphenol Analytical Facility, Montpellier, France.

出版信息

Food Chem. 2022 Aug 15;385:132615. doi: 10.1016/j.foodchem.2022.132615. Epub 2022 Mar 2.

Abstract

Pea protein isolates contain high-quality plant protein. However, they have sensory drawbacks, notably bitterness and astringency, that have limited their use in commercial foods. This study's aim was thus to identify the main phytochemicals in pea-based samples and to examine associations with sensory attributes. The phytochemical profiles of pea flour, pea protein isolates, and pea protein isolate fractions were characterized via UHPLC-DAD-MS. A total of 48 phytochemicals have been revealed: 6 phenolic acids, 5 flavonoids, and 1 saponin were identified and quantified, while another 9 phenolic acids, 10 flavonoids, and 6 saponins were tentatively identified. The impacts of protein extraction and fractionation were studied. These processes appear to have caused some compound degradation. It was found that 29 compounds were correlated with perceived bitterness and/or astringency. Therefore, these results show that certain phytochemicals can lead to negative sensory attributes in pea-protein-based products.

摘要

豌豆分离蛋白含有优质的植物蛋白。然而,它们存在感官缺陷,尤其是苦味和涩味,这限制了它们在商业食品中的应用。因此,本研究的目的是确定豌豆基样品中的主要植物化学物质,并研究其与感官特性的关联。通过超高效液相色谱-二极管阵列检测器-质谱联用仪(UHPLC-DAD-MS)对豌豆粉、豌豆分离蛋白和豌豆分离蛋白组分的植物化学特征进行了表征。共鉴定出48种植物化学物质:6种酚酸、5种黄酮类化合物和1种皂苷被鉴定并定量,另有9种酚酸、10种黄酮类化合物和6种皂苷被初步鉴定。研究了蛋白质提取和分级分离的影响。这些过程似乎导致了一些化合物的降解。发现有29种化合物与感知到的苦味和/或涩味相关。因此,这些结果表明,某些植物化学物质会导致豌豆蛋白基产品产生负面的感官特性。

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