Department of Pediatrics, Alex Ekwueme Federal University Teaching Hospital Abakaliki, Ebonyi State, Nigeria.
Department of Medical Laboratory Science, Alex Ekwueme Federal University Teaching Hospital Abakaliki, Ebonyi State, Nigeria.
Pan Afr Med J. 2022 Jan 5;41:12. doi: 10.11604/pamj.2022.41.12.30535. eCollection 2022.
Tuberculin reactivity following tuberculin skin test which is the most common measure of the effect of the Bacille Calmette Guerin (BCG) vaccination has remained complex. This protocol is aimed to assess the effect of BCG vaccine on tuberculin skin test in Africa as a guide to better understanding or resolving this complexity. A search strategy is developed using MeSH, key words, text words, and entry terms. Five databases will be searched, including PubMed, African Journals Online (AJOL), Google Scholar, Research Gate, and Cochrane Library. Only observational studies conducted in Sub-Saharan Africa and retrievable in English language will be included. The primary measurable outcome of this study is pooled prevalence of positive tuberculin skin tests. Secondary outcomes are factors that influence Tuberculin Skin Test (TST) positivity such as BCG type, the dose of BCG, age at BCG vaccinates, time/interval between BCG vaccination and TST, and type of tuberculin unit used for TST. Identified studies will be screened and selected based on inclusion criteria. Data will be extracted into Zotero, Microsoft Excel and CMA software. Both quality scores and the risk of bias for individual studies will be reported. Studies will be assessed for methodological, clinical, and statistical heterogeneity. Funnel Plots will be used for assessing publication bias. The results will be presented in tabular format in addition to a narrative synthesis. The pooled prevalence of effect of BCG vaccine on TST in Africa will be examined in relation to factors that influence TST positivity.
结核菌素皮肤试验（TST）是评估卡介苗（BCG）疫苗效果的最常用方法，但结果仍然复杂。本方案旨在评估 BCG 疫苗对非洲 TST 的影响，以期更好地理解或解决这一复杂性。我们采用 MeSH、关键词、文本词和入口词制定了检索策略。将对 5 个数据库（包括 PubMed、非洲期刊在线（AJOL）、谷歌学术、Research Gate 和 Cochrane Library）进行检索，仅纳入在撒哈拉以南非洲进行且可检索到英文文献的观察性研究。本研究的主要测量结局是 TST 阳性的汇总患病率。次要结局包括影响 TST 阳性的因素，如 BCG 类型、BCG 剂量、BCG 接种年龄、BCG 接种与 TST 之间的时间/间隔以及 TST 使用的结核菌素单位类型。根据纳入标准筛选和选择符合条件的研究。将数据提取到 Zotero、Microsoft Excel 和 CMA 软件中。将报告单个研究的质量评分和偏倚风险。将评估研究的方法学、临床和统计学异质性。将使用漏斗图评估发表偏倚。结果将以表格形式和叙述性综合呈现，同时还将探讨 TST 阳性影响因素与非洲 BCG 疫苗对 TST 影响的相关性。
