急性脊髓损伤中 miRNA-124、miRNA-544a 和 TNF-α 水平的相关性。
Correlation between miRNA-124, miRNA-544a, and TNF-α levels in acute spinal cord injury.
机构信息
Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, China.
Aab Cardiovascular Research Institute, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
出版信息
Spinal Cord. 2022 Sep;60(9):779-783. doi: 10.1038/s41393-022-00763-4. Epub 2022 Mar 16.
STUDY DESIGN
Retrospective.
OBJECTIVES
Acute spinal cord injury (ASCI) is caused by direct or indirect strikes from external forces on the spinal cord. Here, we investigated the correlation between the miR-124, miR-544a, and TNF-α levels in patients with ASCI, aiming to evaluate the potential usage of miR-124 and miR-544a in ASCI diagnosis.
SETTING
University/hospital.
METHODS
A total of 90 (58 male/32 female) ASIA patients and 15 (9 male/6 female) control patients (with acute limb trauma) were involved in the presented study. The ASIA patients were further subclustered based on the International Standards for the Neurological Classification of SCI (ISNCSCI) exam. 30 (18 male/12 female)cases were determined to have complete spinal cord injury (CSCI) and classified as ASIA grade A (Complete); 30 (20 male/10 female) cases were determined to have incomplete spinal cord injury (ISCI) and classified as ASIA grade B (sensory incomplete), C (motor incomplete), or D (motor incomplete); 30 (20 male/10 female) cases were determined to have normal neurological function (NNF) and classified as ASIA grade E (Normal). Plasma miR-124, miRNA-544a, and tumor necrosis factor-alpha (TNF-α) levels were measured from the blood samples collected 24 h, 48 h, and 72 h after trauma.
RESULTS
The levels of miR-124 and miR-544a in the CSCI and ISCI groups were significantly higher than those of the NNF and the control group 24 h after injury (P < 0.05). The increased levels gradually declined from 24 h to 72 h after injury. The area under the receiver operating characteristic curve (ROC) of miR-124, miR-544a and TNF-α 24 h after trauma in patients with acute spinal cord injury were 0.948 [95% CI (0.890, 1.000)], 0.815 [95% CI (0.638, 0.994)] and 0.770 [95% CI (0.641, 0.879)], respectively.
CONCLUSION
The miRNA-124 and miRNA-544a levels increased significantly in ASCI patients compared with control patients 24 h after injury. These increased levels gradually reduced from 24 h to 72 h after injury. There is a strong positive correlation between miRNA-124, miRNA-544a, and acute spinal cord injury.
SPONSORSHIP
The present study was supported by a University-level project of Ningxia Medical University (Project Number: XY2017147).
研究设计
回顾性研究。
目的
急性脊髓损伤（ASCI）是由脊髓受到外力的直接或间接打击引起的。在这里，我们研究了 ASCI 患者中 miR-124、miR-544a 和 TNF-α 水平之间的相关性，旨在评估 miR-124 和 miR-544a 在 ASCI 诊断中的潜在应用。
地点
大学/医院。
方法
本研究共纳入 90 例（58 例男性/32 例女性）ASIA 患者和 15 例（9 例男性/6 例女性）对照患者（急性肢体创伤）。根据国际脊髓损伤神经分类标准（ISNCSCI）检查，进一步将 ASIA 患者亚组化。30 例（18 例男性/12 例女性）被确定为完全性脊髓损伤（CSCI），并分为 ASIA 分级 A（完全性）；30 例（20 例男性/10 例女性）被确定为不完全性脊髓损伤（ISCI），并分为 ASIA 分级 B（感觉不完全）、C（运动不完全）或 D（运动不完全）；30 例（20 例男性/10 例女性）被确定为神经功能正常（NNF），并分为 ASIA 分级 E（正常）。在创伤后 24、48 和 72 小时采集血液样本，测量血浆 miR-124、miRNA-544a 和肿瘤坏死因子-α（TNF-α）水平。
结果
CSCI 和 ISCI 组伤后 24 小时 miR-124 和 miR-544a 水平明显高于 NNF 组和对照组（P<0.05）。伤后 24 小时至 72 小时，升高的水平逐渐下降。miR-124、miR-544a 和 TNF-α 伤后 24 小时在急性脊髓损伤患者中的受试者工作特征曲线（ROC）下面积分别为 0.948[95%CI（0.890，1.000）]、0.815[95%CI（0.638，0.994）]和 0.770[95%CI（0.641，0.879）]。
结论
与对照组相比，ASCI 患者伤后 24 小时 miR-124 和 miR-544a 水平显著升高。这些升高的水平从伤后 24 小时到 72 小时逐渐降低。miR-124、miR-544a 与急性脊髓损伤之间存在很强的正相关。
资助
本研究由宁夏医科大学大学级项目（项目编号：XY2017147）资助。
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