Beijing Key Laboratory of Environmental and Viral Oncology, Faculty of Environment and Life, Beijing University of Technology, Beijing, China.
J Food Sci. 2022 Apr;87(4):1575-1585. doi: 10.1111/1750-3841.16110. Epub 2022 Mar 15.
In this study, morin magnetic molecularly imprinted polymers (Morin-MMIPs) were synthesized based on magnetic nanoparticles and surface molecularly imprinted technology with superparamagnetism and extraction selectivity. The polymers allowed the separating of morin from complex matrices in the presence of an external magnetic field with no need for centrifugation or filtration. The microstructure of the polymers was characterized by scanning electron microscopy and transmission electron microscopy. Meanwhile, the functional group and magnetic properties of the polymers were characterized using Fourier transform infrared spectroscopy (FT-IR) and magnetic vibration meter (VSM). The maximum adsorption capacity of MMIPs was 3.24 mg/g, which was 2.55 times higher than that of MNIPs (1.27 mg/g). Morin was quantified by HPLC-DAD, which showed good linearity in the concentration range of 0.05-60 µg/ml with the correlation coefficient R = 0.9993. The limit of detection (LOD) was 0.08 µg/ml, and the spiked recoveries were 87.5-106.8%. The calculation of the adsorption isotherm and kinetic model revealed the adsorption mechanisms, and the adsorption process was consistent with the Langmuir adsorption isotherm and pseudo-secondary kinetic models. Likewise, the material has been successfully used to extract and separate morin from food samples. The method reported in this paper has the advantages of fast adsorption speed, high selectivity, and environmental friendliness. It provided a reliable method for the separation and detection of morin or other natural products.
在这项研究中，基于磁性纳米粒子和表面分子印迹技术，合成了具有超顺磁性和萃取选择性的杨梅素磁性分子印迹聚合物（Morin-MMIPs）。该聚合物允许在外磁场的存在下从复杂基质中分离杨梅素，而无需离心或过滤。聚合物的微观结构通过扫描电子显微镜和透射电子显微镜进行了表征。同时，利用傅里叶变换红外光谱（FT-IR）和振动磁强计（VSM）对聚合物的官能团和磁性进行了表征。MMIPs 的最大吸附容量为 3.24mg/g，是 MNIPs（1.27mg/g）的 2.55 倍。采用 HPLC-DAD 对杨梅素进行定量分析，其在 0.05-60μg/ml 的浓度范围内具有良好的线性关系，相关系数 R=0.9993。检测限（LOD）为 0.08μg/ml，加标回收率为 87.5-106.8%。吸附等温线和动力学模型的计算揭示了吸附机制，吸附过程与 Langmuir 吸附等温线和拟二级动力学模型一致。同样，该材料已成功用于从食品样品中提取和分离杨梅素。本文报道的方法具有吸附速度快、选择性高、环境友好等优点，为分离和检测杨梅素或其他天然产物提供了可靠的方法。
