Zhao Tingting, Ye Zhifeng, Liu Yun, Lin Haoran, Li Shuisheng, Zhang Yong
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Am J Physiol Regul Integr Comp Physiol. 2022 May 1;322(5):R454-R465. doi: 10.1152/ajpregu.00307.2021. Epub 2022 Mar 16.
() is a newly identified gene in vertebrates, but its biological functions remain unclear. In this study, we cloned the full-length cDNA of in zebrafish. The 288-bp open reading frame encodes a protein of 95 amino acids that contains a 14 amino acids mature peptide. is highly expressed in brain and testis. Its expression was significantly downregulated in the hypothalamus after feeding treatment and 7 days of food deprivation. Using a zebrafish mutant line, we observed a greater amount of food intake and changes in mRNA levels of feeding factors. We found that, SPX2 acts as a satiety factor that inhibits food intake by downregulating the expression of agouti-related neuropeptide (). Moreover, mutant fish exhibited a larger body size, excessive lipid accumulation, and insulin resistance. Taken together, our results revealed that SPX2 functions as a satiety factor involved in energy metabolic regulation in zebrafish.
（）是脊椎动物中一个新发现的基因，但其生物学功能仍不清楚。在本研究中，我们克隆了斑马鱼中（）的全长cDNA。288bp的开放阅读框编码一个由95个氨基酸组成的蛋白质，该蛋白质包含一个14个氨基酸的成熟肽。（）在脑和睾丸中高表达。喂食处理和禁食7天后，其在下丘脑中的表达显著下调。利用斑马鱼（）突变体系，我们观察到食物摄入量增加以及进食因子mRNA水平的变化。我们发现，SPX2作为一种饱腹感因子，通过下调刺鼠相关神经肽（）的表达来抑制食物摄入。此外，（）突变鱼表现出更大的体型、过多的脂质积累和胰岛素抵抗。综上所述，我们的结果表明SPX2作为一种饱腹感因子参与斑马鱼的能量代谢调节。
