Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
Iran J Immunol. 2022 Mar;19(1):3. doi: 10.22034/IJI.2022.89946.1979.
The methylation of IFN-γ and IL-4 genes is regarded as an epigenetic regulation that maintains the Th1 or Th2 phenotype.
To explore the influence of prenatal administration of the staphylococcal enterotoxin B (SEB) in pregnant rats, on the IFN-γ or IL-4 expression in the offspring spleen.
The SEB or PBS was administered intravenously to pregnant rats on the embryo-day 16. After normal delivery, the spleens from the fifth-day neonates and adult offspring were isolated under anesthesia. Quantitative PCR, western blot, ELISA and MeDIP-qPCR were applied to determine the levels of the splenic IFN-γ or IL-4 mRNAs, their protein levels, and methylation status, respectively.
Prenatal administration of the SEB in pregnant rats decreased the levels of the splenic IFN-γ and IL-4 proteins in neonates, but increased their mRNA levels. However, prenatal administration of the SEB significantly augmented both mRNA and protein levels of the IFN-γ and IL-4 in the adult spleen. In addition, the prenatal SEB administration decreased the methylation of the splenic IFN-γ and IL-4 in adult but not neonatal offspring.
The prenatal administration of SEB in pregnant rats can cause a mixed Th1 and Th2 cytokines response in the offspring spleen, and alter the cytokine expression of the Th1 and Th2 via decreasing the methylation in adult but, not neonatal offspring spleen.
IFN-γ 和 IL-4 基因的甲基化被认为是维持 Th1 或 Th2 表型的表观遗传调控。
探讨产前给予大鼠金黄色葡萄球菌肠毒素 B（SEB）对后代脾 IFN-γ 或 IL-4 表达的影响。
在胚胎第 16 天，将 SEB 或 PBS 经静脉内给予孕鼠。正常分娩后，在麻醉下分离第五天新生儿和成年后代的脾。应用定量 PCR、western blot、ELISA 和 MeDIP-qPCR 分别检测脾 IFN-γ 或 IL-4 mRNA 的水平、其蛋白水平和甲基化状态。
产前给予孕鼠 SEB 降低了新生儿脾 IFN-γ 和 IL-4 蛋白水平，但增加了其 mRNA 水平。然而，产前给予 SEB 显著增加了成年脾中 IFN-γ 和 IL-4 的 mRNA 和蛋白水平。此外，产前 SEB 给药降低了成年而非新生儿后代脾 IFN-γ 和 IL-4 的甲基化。
产前给予孕鼠 SEB 可导致后代脾中混合 Th1 和 Th2 细胞因子反应，并通过降低成年而非新生儿后代脾中的甲基化来改变 Th1 和 Th2 的细胞因子表达。
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