Wei Hongqi, Zhu Zhengwen, Cao Zhongsheng, Liu Zhiyong, Wu Xiaofan, Yuan Hui
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 Sep;28(17):1330-4.
The aim of this study was to determine the expression of GATA-3 and the level of Th1 and Th2 cytokines upon repeated exposure to staphylococcal enterotoxin B(SEB) of different concentrations in the maxillary sinus mucosa of rabbits.
The rabbits were randomly divided into 2 groups (24 rabbits per group): low-dose SEB group and high-dose SEB group. The low-dose SEB group and high-dose SEB group received daily injections of 0.6 ng of SEB (2 ml) and 60 ng of SEB (2 ml) into the left maxillary sinus of rabbits for 28 days, respectively. Concurrent treatment of the right maxillary sinus with normal saline was used as a control. Six rabbits chosen randomly in two groups were killed on days 3, 7, 14, and 28, and to obtain the sinus mucosa from the two-side maxillary sinuses for measurement. Mucosal levels of IL-2, IL-4, IL-5, and IFN-γ were measured using ABC-ELISA. Tissue expression of GATA-3 were examined using Real-time PCR and immunohistochemistry.
IFN-γ and IL-2 levels were significantly elevated in the high-dose SEB group compared with the low-dose SEB and control groups on days 7, 14, and 28 (P < 0.05). However, IL-4 and IL-5 levels were markedly enhanced in the low-dose SEB group compared with the high-dose SEB and control groups on days 14 and 28 (P < 0.05). Real-time PCR showed that the expression of GATA-3 mRNA in the low-dose SEB group was markedly enhanced, and immunohistochemical staining illustrated that the number of GATA-3 positive cells was markedly increased in the low-dose SEB group as compared with the high-dose SEB group (P < 0.05). No significant differences were observed in GATA-3 expression between the high-dose SEB and the control groups (P > 0.05).
SEB promoted Th1 cytokines production at high concentrations, and enhanced Th2 cytokines expression and Th2 immune response at low concentrations.
本研究旨在确定兔上颌窦黏膜反复暴露于不同浓度葡萄球菌肠毒素B（SEB）后GATA-3的表达以及Th1和Th2细胞因子的水平。
将兔随机分为2组（每组24只）：低剂量SEB组和高剂量SEB组。低剂量SEB组和高剂量SEB组分别每天向兔左侧上颌窦注射0.6 ng SEB（2 ml）和60 ng SEB（2 ml），共28天。右侧上颌窦用生理盐水同时处理作为对照。两组中随机选取6只兔，分别在第3、7、14和28天处死，获取双侧上颌窦的窦黏膜进行检测。采用ABC-ELISA法检测黏膜中IL-2、IL-4、IL-5和IFN-γ的水平。采用实时定量PCR和免疫组织化学法检测GATA-3的组织表达。
与低剂量SEB组和对照组相比，高剂量SEB组在第7、14和28天IFN-γ和IL-2水平显著升高（P<0.05）。然而，与高剂量SEB组和对照组相比，低剂量SEB组在第14和28天IL-4和IL-5水平明显升高（P<0.05）。实时定量PCR显示，低剂量SEB组GATA-3 mRNA表达明显增强，免疫组织化学染色显示，与高剂量SEB组相比，低剂量SEB组GATA-3阳性细胞数量明显增加（P<0.05）。高剂量SEB组与对照组之间GATA-3表达无显著差异（P>0.05）。
高浓度SEB促进Th1细胞因子产生，低浓度SEB增强Th2细胞因子表达和Th2免疫反应。
