基于细胞的测定法，以鉴定 Nrf2/ARE 通路的调节剂。
Cell-Based Assays to Identify Modulators of Nrf2/ARE Pathway.
机构信息
National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2022;2474:59-69. doi: 10.1007/978-1-0716-2213-1_7.
Abstract
The nuclear factor erythroid 2-related factor (Nrf2) and antioxidant response element (ARE) signaling pathway play an important role in the amelioration of cellular oxidative stress. Thus, assays that detect this pathway can be useful for identifying chemicals that induce or inhibit oxidative stress signaling. This chapter is to describe two cell-based Nrf2/ARE assays in a quantitative high-throughput screening (HTS) format to test a large collection of chemicals for oxidative stress induction ability. The assay descriptions involve cell handling, assay preparation, instrument usage, and assay procedure.
摘要
核因子红细胞 2 相关因子 (Nrf2) 和抗氧化反应元件 (ARE) 信号通路在改善细胞氧化应激方面发挥着重要作用。因此，检测该通路的测定方法可用于鉴定诱导或抑制氧化应激信号的化学物质。本章描述了两种基于细胞的 Nrf2/ARE 测定方法，以定量高通量筛选 (HTS) 格式测试大量化学物质的氧化应激诱导能力。测定方法描述涉及细胞处理、测定准备、仪器使用和测定程序。
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