Aquaporins Group, Centro de Edafología y Biología Aplicada del Segura, CEBAS-CSIC, Campus de Espinardo - 25, E-30100 Murcia, Spain; Phytochemistry and Healthy Food Lab (LabFAS), Department of Food Science Technology, Centro de Edafología y Biología Aplicada del Segura, CEBAS-CSIC, Campus de Espinardo-25, E-30100 Murcia, Spain.
Phytochemistry and Healthy Food Lab (LabFAS), Department of Food Science Technology, Centro de Edafología y Biología Aplicada del Segura, CEBAS-CSIC, Campus de Espinardo-25, E-30100 Murcia, Spain.
Food Chem. 2022 Aug 15;385:132680. doi: 10.1016/j.foodchem.2022.132680. Epub 2022 Mar 10.
Isothiocyanates (ITCs) have low stability in aqueous conditions, reducing their bioavailability when used as food ingredients. Therefore, the aim of this work was to increase the stability of the ITCs present in extracts of Bimi® edible parts by nanoencapsulation using cauliflower-derived plasma membrane vesicles. The bioactivity of these nanoencapsulates was evaluated in a HepG2 hepatocyte cell line in a model for low-grade chronic inflammation. The vesicles showed a higher capacity of retention in the in vitro gastrointestinal digestion for 3,3-diindolylmethane (DIM), indole-3-carbinol (I3C) and sulforaphane (SFN). Furthermore, Transmission Electron Microscopy (TEM) analysis of the vesicles revealed a decreased size under acidic pH and a release of their cargo after the intestinal digestion. The HepG2 experiments revealed differences in metabolism under the condition of chronic inflammation. The cauliflower-derived plasma membrane vesicles are able to enhance the stability of ITCs through the in vitro gastrointestinal digestion, improving their bioaccesibility and potential bioavailability.
异硫氰酸酯（ITCs）在水介质中稳定性差，作为食品成分使用时其生物利用度降低。因此，本工作旨在通过使用花椰菜衍生质膜囊泡对 Bimi®可食用部分提取物中的 ITC 进行纳米封装，来提高其稳定性。在低水平慢性炎症模型中，用 HepG2 肝细胞系评估这些纳米囊泡的生物活性。囊泡在体外胃肠道消化中对 3,3-二吲哚甲烷（DIM）、吲哚-3-甲醇（I3C）和萝卜硫素（SFN）的保留能力更高。此外，囊泡的透射电子显微镜（TEM）分析显示，在酸性 pH 下其粒径减小，并且在肠道消化后释放其货物。HepG2 实验揭示了在慢性炎症条件下代谢的差异。花椰菜衍生质膜囊泡能够通过体外胃肠道消化提高 ITCs 的稳定性，从而改善其生物利用度和潜在生物利用度。
