儿童发病多发性硬化及相关脱髓鞘疾病患者肠道微生物组的稳定性。
Stability of the gut microbiota in persons with paediatric-onset multiple sclerosis and related demyelinating diseases.
机构信息
Division of Neurology, Faculty of Medicine, The University of British Columbia, Vancouver, BC, Canada/The Djavad Mowafaghian Centre for Brain Health, Vancouver, BC, Canada.
Center for Neuroinflammation and Experimental Therapeutics and Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
出版信息
Mult Scler. 2022 Oct;28(11):1819-1824. doi: 10.1177/13524585221079533. Epub 2022 Mar 16.
OBJECTIVE
Examine if the gut microbiota composition changes across repeated samples in paediatric-onset multiple sclerosis (MS) or monophasic-acquired demyelinating syndromes (monoADS).
METHODS
A total of 36 individuals (18 MS/18 monoADS) with ⩾2 stool samples were included. Stool sample-derived DNA was sequenced. Alpha/beta diversities and genus-level taxa were analysed.
RESULTS
Mean ages at first sample procurement (MS/monoADS) = 18.0/13.8 years. Median time (months) between first/second samples = 11.2 and second/third = 10.3. Alpha/beta diversities did not differ between stool samples ( > 0.09), while one genus - did ( = 0.001).
CONCLUSIONS
The gut microbiota composition in paediatric-onset MS and monoADS exhibited stability, suggesting that single stool sample procurement is a reasonable first approach.
目的
研究儿科发病的多发性硬化症（MS）或单相获得性脱髓鞘综合征（monoADS）患者的粪便样本中肠道微生物组成是否随重复样本发生变化。
方法
共纳入 36 名个体（18 名 MS/18 名 monoADS，均有≥2 份粪便样本）。对粪便样本中的 DNA 进行测序。分析 alpha/beta 多样性和属水平分类群。
结果
首次取样时的平均年龄（MS/monoADS）分别为 18.0/13.8 岁。首次/第二次取样之间的中位数时间（月）为 11.2，第二次/第三次为 10.3。粪便样本之间的 alpha/beta 多样性无差异（>0.09），但有一个属存在差异（=0.001）。
结论
儿科发病的 MS 和 monoADS 患者的肠道微生物组成表现出稳定性，这表明单次粪便样本采集是一种合理的初步方法。
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