Mendes Talita Biude, Simas Joana Noguères, Fischer Leonardo Wensing, Paccola Camila Cicconi, de Oliva Samara Urban, Vendramini Vanessa, Miraglia Sandra Maria
Laboratory of Reproduction and Developmental Biology (LaBReD), Department of Morphology and Genetics, Federal University of Sao Paulo, UNIFESP, Sao Paulo, Brazil.
Andrologia. 2022 Jul;54(6):e14417. doi: 10.1111/and.14417. Epub 2022 Mar 16.
In varicocele, the main cause of sperm DNA damage is oxidative stress (OS). Resveratrol, a polyphenol with antioxidant properties, can protect cells from injuries caused by OS. We investigated the benefits of resveratrol against reproductive damage caused by experimental varicocele induced from peripuberty. Eighty peripubertal male rats were distributed into 4 groups: sham-control (S), varicocele (V), resveratrol (R) and varicocele treated with resveratrol (VR). Varicocele was induced through the partial ligature of the left renal vein. Resveratrol was given in a daily dose of 300 mg/kg body weight (gavage). Sperm samples were collected at 100 days of age for vitality, DNA fragmentation and chromatin protamination evaluations. OS analyses were carried out. Rats from all groups were mated with healthy primiparous females for evaluation of reproductive capacity and embryonic quality. The V group showed reduction of sperm vitality, altered chromatin protamination and sperm DNA integrity and high levels of OS. The VR group showed an improvement of oxidative status, sperm vitality, DNA integrity and chromatin structure, and an enhancement in the gestational index and embryonic quality. Therefore, we showed in this experimental model that resveratrol is a promising nutraceutical adjuvant and should be deeply studied to mitigate subfertility in varicocele.
在精索静脉曲张中，精子DNA损伤的主要原因是氧化应激（OS）。白藜芦醇是一种具有抗氧化特性的多酚，可保护细胞免受OS造成的损伤。我们研究了白藜芦醇对青春期前实验性精索静脉曲张所致生殖损伤的益处。80只青春期前雄性大鼠被分为4组：假手术对照组（S）、精索静脉曲张组（V）、白藜芦醇组（R）和白藜芦醇治疗的精索静脉曲张组（VR）。通过部分结扎左肾静脉诱导精索静脉曲张。白藜芦醇以每日300mg/kg体重的剂量灌胃给药。在100日龄时采集精子样本，进行活力、DNA片段化和染色质鱼精蛋白化评估，并进行OS分析。将所有组的大鼠与健康初产雌性交配以评估生殖能力和胚胎质量。V组精子活力降低，染色质鱼精蛋白化改变，精子DNA完整性受损，且OS水平较高。VR组氧化状态、精子活力、DNA完整性和染色质结构得到改善，妊娠指数和胚胎质量提高。因此，我们在这个实验模型中表明，白藜芦醇是一种有前景的营养辅助剂，应深入研究以减轻精索静脉曲张患者的生育力低下问题。
