CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Centre of Biological Engineering (CEB), Laboratório de Investigação em Biofilmes Rosário Oliveira, University of Minho, Braga, Portugal.
FEMS Microbiol Ecol. 2022 Apr 6;98(4). doi: 10.1093/femsec/fiac031.
Within the oral cavity, the ability of Candida species to adhere and form biofilms is well-recognized, especially when Candida albicans is considered. Lately, a knowledge gap has been identified regarding dual-species communication of Candida isolates, as a way to increase virulence, with evidences being collected to support the existence of interactions between C. albicans and Candida parapsilosis. The present work evaluated the synergistic effect of the two Candida species, and explored chemical interactions between cells, evaluating secreted extracellular alcohols and their relation with yeasts' growth and matrix composition. A total of four clinical strains of C. albicans and C. parapsilosis species, isolated from single infections of different patients or from co-infections of a same patient, were tested. It was found that dual-species biofilms negatively impacted the growth of C. parapsilosis and their biofilm matrix, in comparison with mono-species biofilms, and had minor effects on the biofilm biomass. Alcohol secretion revealed to be species- and strain-dependent. However, some dual-species cultures produced much higher amounts of some alcohols (E-nerolidol and E, E-Farnesol) than the respective single cultures, which proves the existence of a synergy between species. These results show evidence that interactions between Candida species affect the biofilm matrix, which is a key element of oral biofilms.
在口腔内，念珠菌属物种的黏附并形成生物膜的能力是众所周知的，尤其是当考虑到白色念珠菌时。最近，人们发现了关于念珠菌分离株双物种间通讯的知识空白，这是一种增加毒力的方式，有证据表明白色念珠菌和近平滑念珠菌之间存在相互作用。本研究评估了这两种念珠菌的协同作用，并通过评估细胞分泌的细胞外醇及其与酵母生长和基质组成的关系，探索了细胞间的化学相互作用。共测试了来自不同患者单一致病菌或同一患者混合感染的四种临床分离的白色念珠菌和近平滑念珠菌。结果发现，与单物种生物膜相比，双物种生物膜对近平滑念珠菌的生长及其生物膜基质有负面影响，而对生物膜生物量的影响较小。醇的分泌表现出物种和菌株依赖性。然而，一些双物种培养物产生的某些醇（E-橙花叔醇和 E，E-法呢醇）的量明显高于各自的单培养物，这证明了物种之间存在协同作用。这些结果表明，念珠菌物种之间的相互作用会影响生物膜基质，这是口腔生物膜的关键要素。
