In vitro and in vivo anthelmintic effects of (Cham-Holl) leaf extract against in experimentally infected broiler chickens.

This study was to assess the anthelminthic potential of leaf extract against in experimentally infected broiler chickens. The extract and fractions were evaluated for in vitro inhibition and in vivo anthelmintic effects. Acute toxicity studies of extract revealed no sign of toxicity or death in birds at oral dose range of 1000-5000 and was considered safe. There was a concentration dependent decrease on inhibition of egg embryonation and deparasitization. At 100 mg/ml, albendazole (ALB) caused the highest inhibition of embryonation (195.3 ± 0.9) which was not significantly different from the decrease caused by crude methanol extract (CME) (188 ± 0.9), hexane fraction (HF) (177 ± 1.2) or ethyl acetate fraction (EAF) (168.3 ± 0.9). The highest inhibition rates (%) were 97, 94, 88 and 85 for ALB, CME, HF and EAF, respectively. The deparasitization obtained at day 21 in ALB (95.62%) treated birds was not significantly ( > 0.05) different from the 81.27% and 89.24% obtained from the crude methanol treated birds. The deparasitization caused by CME at 400 mg/kg (89.24%) was significantly higher than the one caused by EAF at the same dose (50.19%). Day 21 post treatment, significantly ( < 0.05) higher deparasitization was recorded for CME and HF at dosage of 400 mg/kg when compared to 200 mg/kg. Histopathology findings revealed necrosis of the mucosal gland and villi in chickens. In conclusion, the leaf extract and fractions have been shown to possess anthelmintic activity.