伊朗西南部屠宰绵羊中 和 属（肉孢子虫科：顶复门）的检测与系统发育分析。
Detection and phylogenetic analysis of and spp. (Sarcocystidae: Apicomplexa) from slaughtered sheep in southwest Iran.
作者信息
Shahabi Saeed, Dehbashi Nima, Sarkari Bahador, Arefkhah Nasir, Sedaghat Bahareh, Savardashtaki Amir
机构信息
Department of Medical Entomology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
J Parasit Dis. 2022 Mar;46(1):215-219. doi: 10.1007/s12639-021-01433-7. Epub 2021 Aug 16.
species are intracellular protozoan which mostly complete their life cycle in two hosts. The parasite has a significant economic, medical and veterinary impact in many regions of the world and considered as a significant health problem in Iran. However, most of infections are asymptomatic and mortality is extremely rare. The present study aimed to determine the molecular phylogeny of the species isolated from sheep slaughtered in southwest Iran, using mitochondrial DNA sequences of 18 S rRNA gene. The DNA was extracted from sheep muscular tissue (n = 60), and partial sequence of 18 S rDNA was amplified and sequenced. Phylogenetic analysis revealed two monophyletic clades representing (n = 3) and spp. (n = 3). BI posterior probability and MP bootstrap values strongly supported the monophyly of these clades. In conclusion, phylogenetic analysis of species using 18 S rRNA gene could be helpful in identifying the new species of the .
该物种是细胞内原生动物，大多在两个宿主中完成其生命周期。这种寄生虫在世界许多地区都有重大的经济、医学和兽医影响，在伊朗被视为一个重大的健康问题。然而，大多数感染是无症状的，死亡率极其罕见。本研究旨在利用18S rRNA基因的线粒体DNA序列，确定从伊朗西南部屠宰的绵羊中分离出的该物种的分子系统发育。从绵羊肌肉组织（n = 60）中提取DNA，并扩增和测序18S rDNA的部分序列。系统发育分析揭示了两个单系分支，分别代表（n = 3）和 spp.（n = 3）。贝叶斯推断后验概率和最大简约法自展值强烈支持这些分支的单系性。总之，利用18S rRNA基因对该物种进行系统发育分析有助于鉴定该物种的新物种。
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