Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Comp Immunol Microbiol Infect Dis. 2022 Jan;80:101738. doi: 10.1016/j.cimid.2021.101738. Epub 2021 Dec 13.
Sarcocystis is an intracellular parasite of the apicomplexa phylum. More than one hundred species of Sarcocystis can infect wildlife and livestock animals. Samples of the liver, heart, muscle and diaphragm were collected from sheep, goats and cattle in Gonabad, northeast Iran and subjected to macroscopic, microscopic, tissue digestion, sequencing and phylogenetic analyses of 18S-rRNA region. Tissue sections stained with hematoxylin and eosin were also provided for surveying sarcocysts in the samples. Tissue digestion showed that all animal samples (100%) were infected with Sarcocystis bradyzoites. Phylogenetic analysis indicated that out of 50% of sheep genotypes belonged to S. tenella, and 20% to S. moulei and S. arieticanis. Moreover, three samples of macroscopic specimens of sheep were identified as S. gigantea, 100% of cattle isolates infected with S. cruzi, 80% of goat isolates belonged to S. capracanis and one macroscopic specimen of goat (20%) identified as S. moulei. Sarcocystis infection is highly prevalent in livestock in northeast Iran, where a variety of Sarcocystis species are unequivocally circulating in the region.
肉孢子虫是顶复门的一种细胞内寄生虫。超过一百种的肉孢子虫可以感染野生动物和家畜动物。本研究从伊朗东北部贡纳巴德的绵羊、山羊和牛采集肝脏、心脏、肌肉和横膈膜样本，并进行了宏观、微观、组织消化、18S-rRNA 区测序和系统发育分析。还提供了用苏木精和曙红染色的组织切片，以调查样本中的肉孢子虫。组织消化显示，所有动物样本（100%）都感染了肉孢子虫缓殖子。系统发育分析表明，50%的绵羊基因型属于 S. tenella，20%属于 S. moulei 和 S. arieticanis。此外，3 份绵羊宏观样本被鉴定为 S. gigantea，100%的牛分离株感染 S. cruzi，80%的山羊分离株属于 S. capracanis，1 份山羊宏观样本（20%）鉴定为 S. moulei。在伊朗东北部，家畜肉孢子虫感染非常普遍，该地区明确存在多种肉孢子虫种循环。
