Pei Yangli, Fan Ziyao, Song Yuxin, Chen Chujie, Mu Yulian, Li Bugao, Feng Zheng, Li Hua, Li Kui
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan University, Foshan, China.
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Genet. 2022 Mar 1;13:764965. doi: 10.3389/fgene.2022.764965. eCollection 2022.
Myostatin (MSTN) is a protein that negatively regulates growth of skeletal muscle, and inactivation of MSTN improves the mass of skeletal muscle. Our previous work found that pigs have higher muscle depth and lower fat depth compared to wild type without any developmental problems. Therefore, -edited pigs are most likely to appear as heterozygotes in the potential future market, but the characteristics of organs in digestive and reproductive system of pigs with gene editing remains unclear. Here, we investigated the histological of the organs in the digestive system and reproductive system in gene heterozygotes at adult stages. The length of intestine was further compared between adult heterozygous and wild type pigs. We found no significant differences in histomorphology of organs, including heart, duodenum, jejunum, ileum, cecum, colon, testis, epididymis, ovaries, oviducts and uterus, between individuals from two genotypes. Moreover, there was no significant difference in the average length of intestine in adult pigs. Our data provide a reference for further clarifying the applications of gene edited pigs.
肌生成抑制素（MSTN）是一种对骨骼肌生长起负向调节作用的蛋白质，MSTN失活可增加骨骼肌质量。我们之前的研究发现，与野生型相比，[基因编辑的]猪具有更高的肌肉厚度和更低的脂肪厚度，且没有任何发育问题。因此，在未来潜在市场中，[基因编辑的]猪很可能以杂合子形式出现，但基因编辑猪消化系统和生殖系统器官的特征仍不清楚。在此，我们研究了成年阶段[基因编辑]杂合子猪消化系统和生殖系统器官的组织学情况。进一步比较了成年杂合子猪和野生型猪的肠道长度。我们发现，两种基因型个体的心脏、十二指肠、空肠、回肠、盲肠、结肠、睾丸、附睾、卵巢、输卵管和子宫等器官的组织形态没有显著差异。此外，成年猪的平均肠道长度也没有显著差异。我们的数据为进一步阐明基因编辑猪的应用提供了参考。
