Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China.
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China.
Anal Chim Acta. 2022 Apr 8;1201:339639. doi: 10.1016/j.aca.2022.339639. Epub 2022 Feb 21.
A novel approach for measurement of alpha-fetoprotein (AFP) was developed with the detection of inductively coupled plasma mass spectrometry (ICP-MS), where lanthanide nanoparticles (NaYF: Yb, Er) were employed as immunolabeling probes. Amino modified NaYF: Yb, Er nanoparticles were synthesized via the hydrothermal procedure, which was followed by conjugated with anti-AFP McAb (labeling, Ab) to specifically identify AFP as immunolabeling probes. Y in immunolabeling probes was employed as an elemental tag for AFP measurement with ICP-MS, along with a linear range of 2-180 ng mL, and a LOD of 0.44 ng mL, human serum was employed to validate the approach. Cells imaging was accomplished with laser ablation (LA)-ICP-MS to confirm the combination of cancer cells and the probes. Furthermore, Y of the probes were used as an elemental tag for cancer cells counting in clinical blood samples with time resolved (TRA)-ICP-MS, indicating the potential of lanthanide nanoparticles in clinical diagnosis.
开发了一种新的方法来测量甲胎蛋白（AFP），该方法采用电感耦合等离子体质谱（ICP-MS）检测，其中使用了镧系纳米粒子（NaYF: Yb, Er）作为免疫标记探针。通过水热法合成了氨基修饰的 NaYF: Yb, Er 纳米粒子，然后与抗 AFP McAb（标记，Ab）结合，以特异性识别 AFP 作为免疫标记探针。在 ICP-MS 中，探针中的 Y 被用作 AFP 测量的元素标记物，线性范围为 2-180ng mL，LOD 为 0.44ng mL，用人血清验证了该方法。采用激光烧蚀（LA）-ICP-MS 进行细胞成像，以确认癌细胞与探针的结合。此外，探针中的 Y 还被用作时间分辨（TRA）-ICP-MS 中临床血液样本中癌细胞计数的元素标记物，表明镧系纳米粒子在临床诊断中的潜力。
